Hayashi K, Nieckarz J A, Thabit G, Bogdanske J J, Cooley A J, Markel M D
Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA.
Lasers Surg Med. 1997;20(2):164-71. doi: 10.1002/(sici)1096-9101(1997)20:2<164::aid-lsm7>3.0.co;2-o.
The nonablative application of holmium:yttrium-aluminum-garnet (Ho:YAG) laser energy to the joint capsule of patients with glenohumeral instability has been found to shrink capsular tissue and to help stabilize the joint. The purpose of this study was to evaluate the effect of nonablative laser energy on the short-term histological properties of joint capsular tissue in an in vivo rabbit model.
STUDY DESIGN/MATERIALS AND METHODS: Eighteen mature New Zealand white rabbits were used in this study. One randomly selected stifle was treated with laser energy, and the contralateral stifle was sham-operated. Animals were euthanized immediately after surgery (day 0), at 7 days postsurgery and 30 days postsurgery. Specimens were processed for histology and transmission electron microscopy.
Laser-treated samples at day 0 showed diffuse hyalinization of collagen with nuclear karyorrhexis of fibroblasts. Laser-treated tissue at 7 days postsurgery revealed fibroblast proliferation around and into acellular hyalinized regions of collagen. At 30 days postlaser treatment, areas of fused collagen were greatly reduced as large reactive fibroblasts migrated and secreted matrix.
This study illustrates the short-term in vivo tissue response to nonablative laser treatment, where acellular hyalinized regions of collagen are infiltrated by fibroblasts that have used the treated collagen as the framework for migration and secretion of new collagen matrix in order for tissue repair to proceed.
已发现将钬：钇铝石榴石（Ho:YAG）激光能量非消融性应用于肩肱关节不稳患者的关节囊，可使囊组织收缩并有助于稳定关节。本研究的目的是在体内兔模型中评估非消融性激光能量对关节囊组织短期组织学特性的影响。
研究设计/材料与方法：本研究使用了18只成年新西兰白兔。随机选择一侧膝关节接受激光能量治疗，对侧膝关节进行假手术。动物在术后立即（第0天）、术后7天和术后30天实施安乐死。对标本进行组织学和透射电子显微镜检查。
第0天的激光治疗样本显示胶原弥漫性玻璃样变，伴有成纤维细胞核固缩。术后7天的激光治疗组织显示成纤维细胞在胶原无细胞玻璃样变区域周围及内部增殖。激光治疗后30天，随着大量反应性成纤维细胞迁移并分泌基质，融合胶原区域大幅减少。
本研究阐明了非消融性激光治疗在体内的短期组织反应，即胶原无细胞玻璃样变区域被成纤维细胞浸润，这些成纤维细胞以经处理的胶原为迁移和分泌新胶原基质的框架，从而促进组织修复。
