Hayashi K, Thabit G, Vailas A C, Bogdanske J J, Cooley A J, Markel M D
Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison 53706, USA.
Am J Sports Med. 1996 Sep-Oct;24(5):640-6. doi: 10.1177/036354659602400513.
The purpose of this study was to evaluate the effect of laser energy at nonablative levels on joint capsular histologic and biochemical properties in an in vitro rabbit model. The medial and lateral portions of the femoropatellar joint capsule from both stifles of 12 mature New Zealand White rabbits were used. Specimens were divided into three treatment groups (5 watts, 10 watts, and 15 watt) and one control group using a randomized block design. Specimens were placed in a 37 degrees bath of lactated Ringer's solution and laser energy was applied using a holmium:yttrium-aluminum-garnet laser in four transverse passes across the tissue at a velocity of 2 mm/sec with the handpiece set 1.5 mm from the synovial surface. Histologic analysis revealed thermal alteration of collagen (fusion) and fibroblasts (pyknosis) at all energy densities, with higher laser energy causing significantly greater morphologic changes over a larger area (P < 0.05). Application of laser energy did not significantly alter the biochemical parameters evaluated, including type I collagen content and nonreducible crosslinks (P > 0.05). This study demonstrated that nonablative laser energy caused significant thermal damage to the joint capsular tissue in an energy-dependent fashion, but type I collagen content and nonreducible crosslinks (P > 0.05). This study demonstrated that nonablative laser energy caused significant thermal damage to the joint capsular tissue in an energy-dependent fashion, but type I Collagen content and nonreducible corsslinks were not significantly altered.
本研究的目的是在体外兔模型中评估非消融水平的激光能量对关节囊组织学和生化特性的影响。使用了12只成年新西兰白兔双侧膝关节股骨髌骨关节囊的内侧和外侧部分。采用随机区组设计将标本分为三个治疗组（5瓦、10瓦和15瓦）和一个对照组。将标本置于37℃的乳酸林格氏液浴中，使用钬:钇铝石榴石激光以2毫米/秒的速度在组织上横向照射四次，手持探头距滑膜表面1.5毫米施加激光能量。组织学分析显示，在所有能量密度下，胶原蛋白（融合）和成纤维细胞（核固缩）均有热改变，激光能量越高，在更大面积上引起的形态学变化越显著（P<0.05）。激光能量的施加并未显著改变所评估的生化参数，包括I型胶原蛋白含量和不可还原交联（P>0.05）。本研究表明，非消融激光能量以能量依赖的方式对关节囊组织造成显著热损伤，但I型胶原蛋白含量和不可还原交联未显著改变。
