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柔嫩艾美耳球虫小配子发生与受精的扫描电子显微镜观察

Scanning electron microscopy of Eimeria tenella microgametogenesis and fertilization.

作者信息

Madden P A, Vetterling J M

出版信息

J Parasitol. 1977 Aug;63(4):607-10.

PMID:886403
Abstract

Microgametes of Eimeria tenella were observed in cecal tissue from infected chicks killed six days after inoculation with oocysts. Development from the microgamont stage to maturity was followed. We observed three commonly found, but distinctly different, stages in developing microgametes while they were still associated with microgamonts. In the first, only the flagella could be seen protruding through the enveloping microgamont layer. In the second, both the bodies and flagella could be seen; the flagella had increased in size, and the bodies appeared bulbous. In the third, nearly mature microgametes were randomly arranged around the microgamont, and the manner of flagellar attachment to the bodies near the apical conjunction of the perforatorium was seen. A free microgamete and a macrogamete with one penetrating and two adhering microgametes were found. On flagellum and most of the body of the penetrating microgamete had entered the macrogamete. All micorgametes seen had two flagella.

摘要

在接种卵囊六天后处死的感染雏鸡的盲肠组织中观察到柔嫩艾美耳球虫的小配子。追踪了从小配子体阶段到成熟阶段的发育过程。我们观察到正在发育的小配子在仍与小配子体相连时通常会出现的三个不同阶段。在第一个阶段,只能看到鞭毛从包围小配子体的层中伸出。在第二个阶段,可以看到小配子体的主体和鞭毛;鞭毛变大,主体呈球形。在第三个阶段,接近成熟的小配子围绕小配子体随机排列,并且可以看到鞭毛在穿孔器顶端连接处附近与主体的附着方式。发现了一个游离的小配子以及一个带有一个穿透和两个附着小配子的大配子。穿透的小配子的一条鞭毛和大部分主体已经进入大配子。观察到的所有小配子都有两条鞭毛。

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