Plebani M, Borghesan F, Bernardi D, Faggian D
Department of Laboratory Medicine, University-Hospital, Padova, Italy.
Eur J Clin Chem Clin Biochem. 1996 Jul;34(7):579-84.
We studied the analytical and clinical efficiency of a "second generation" technique for the in vitro determination of specific IgE, the so-called CARLA system (Capture Assay Radim Liquid Allergen). Reproducibility studies demonstrated satisfactory intra- and inter-assay analytical imprecision. The linearity test gave good results, and no significant interference by non-specific total IgE was found. The diagnostic efficiency of the system for the five most representative aeroallergens (D1, Dermatophagoides pteronyssinus; E1, Cat's epithelium; G5, Lolium perenne; W19, Parietaria officinalis and W5, Arthemisia absinthium) was evaluated using the Receiver Operating Characteristic (ROC) analysis in eighty-nine patients with suspected inhalant allergies. For each aeroallergen the area under the ROC curve and the most efficient threshold were calculated. Satisfactory areas under the ROC curve were found for D1, G5, E1 and W19, while a lower diagnostic efficiency was observed for W5. The most efficient thresholds differed from one allergen to another, although the differences were not great.
我们研究了一种用于体外测定特异性IgE的“第二代”技术，即所谓的CARLA系统（Capture Assay Radim Liquid Allergen）的分析效率和临床效率。重复性研究表明，该方法在批内和批间分析中具有令人满意的不精密度。线性测试结果良好，未发现非特异性总IgE的显著干扰。使用受试者工作特征（ROC）分析，对89例疑似吸入性过敏患者评估了该系统对五种最具代表性的气传变应原（D1，屋尘螨；E1，猫上皮；G5，黑麦草；W19，药用墙草和W5，苦艾）的诊断效率。计算了每种气传变应原的ROC曲线下面积和最有效的阈值。D1、G5、E1和W19的ROC曲线下面积令人满意，而W5的诊断效率较低。尽管差异不大，但最有效的阈值因变应原而异。
