Islam M N, Kornberg L, Veenker E, Cohen D M, Bhattacharyya I
Department of Oral Pathology, Medicine and Radiology, School of Dentistry, Indiana University, Indianapolis, IN, USA.
Head Neck Pathol. 2010 Mar;4(1):10-4. doi: 10.1007/s12105-009-0151-0. Epub 2009 Nov 24.
The location of oral leukoplakia correlates strongly with the probability of finding dysplastic or malignant alterations at biopsy. It is well established that early detection can dramatically improve the 5-year survival rates for oral squamous cell carcinomas. Since aneuploidy is predictive of future conversion to malignancy, we hypothesized that dysplastic lesions from high-risk sites (floor of mouth, tongue and lips) would exhibit greater aneuploidy than low-risk sites (palate, gingiva and buccal mucosa). Epithelial sections from 60 archival samples diagnosed as mild dysplasia (36 females, 20 males) from various high/low risk locations were stained with Blue Feulgen Stain for DNA Ploidy Analysis (Clarient, Aliso Viejo, CA) and ploidy was analyzed using a ChromaVision ACIS II (Clarient, ALiso Viejo, CA) Image cytometry system. A DNA histogram was generated using an image analyzing software that evaluated the amount of Feulgen stain which is proportional to the amount of nuclear DNA. An ANOVA analysis followed by the Student's't' test revealed significant differences between means (P <or= 0.05). Lesions originating from lateral/ventral tongue (85%), floor of mouth (50%) and soft palate (44%) exhibited a higher frequency of aneuploidy than lesions from gingiva (22%) and lower lip (25%). This pilot study demonstrates that dysplastic lesions from high-risk sites such as the floor of the mouth and lateral/ventral tongue have higher frequency of aneuploidy.
口腔白斑的发病部位与活检时发现发育异常或恶性病变的可能性密切相关。众所周知，早期检测可显著提高口腔鳞状细胞癌的5年生存率。由于非整倍体可预测未来恶变情况，我们推测来自高危部位（口底、舌和唇）的发育异常病变比低危部位（腭、牙龈和颊黏膜）表现出更高的非整倍体率。对60份存档样本（36名女性，20名男性）的上皮切片进行诊断，这些样本来自不同高低危部位，均被诊断为轻度发育异常，用蓝色福尔根染色进行DNA倍体分析（Clarient公司，加利福尼亚州阿利索维耶霍），并使用ChromaVision ACIS II（Clarient公司，加利福尼亚州阿利索维耶霍）图像细胞仪系统分析倍体。使用图像分析软件生成DNA直方图，该软件评估与核DNA量成比例的福尔根染色量。方差分析后进行学生t检验，结果显示均值之间存在显著差异（P≤0.05）。起源于舌外侧/腹侧（85%）、口底（50%）和软腭（44%）的病变比牙龈（22%）和下唇（25%）的病变表现出更高的非整倍体率。这项初步研究表明，来自口底和舌外侧/腹侧等高危部位的发育异常病变具有更高的非整倍体率。
