Watkins B A, Shen C L, Allen K G, Seifert M F
Department of Food Science, Purdue University, West Lafayette, Indiana, USA.
J Bone Miner Res. 1996 Sep;11(9):1321-32. doi: 10.1002/jbmr.5650110917.
This study examined the effects of dietary (n-6) and (n-3) polyunsaturated fatty acids (PUFA) and acetylsalicylic acid (ASA) on bone ash content, morphometry, fatty acid composition, ex vivo PGE2 biosynthesis, tissue IGF-I concentration, and serum alkaline phosphatase (ALPase) activity in chicks. Newly hatched chicks were fed a semipurified diet containing soybean oil (S) or menhaden oil / safflower oil (M) at 90 g/kg. At 4 days of age, chicks were divided into four equal treatment groups receiving 0 mg [symbol: see text] or 500 mg [symbol: see text] of ASA/kg of diet: S[symbol: see text]ASA, M[symbol: see text]ASA, S[symbol: see text]ASA, and M[symbol: see text]ASA. Lipid and ASA treatments did not affect bone length, bone ash, or bone mineral content in chicks. Chicks fed M had increased fractional labeled trabecular surface and tissue level bone formation rates, independent of ASA treatment, compared with those given S. A significant fat x ASA interaction effect was found for trabecular bone volume, thickness, separation, and number. Chicks fed S had higher 20:4(n-6) but lower 20:5(n-3) concentrations in liver and bone compared with those given M. Ex vivo PGE2 biosynthesis was higher in liver homogenates and bone organ cultures of chicks fed S compared with the values for those given M at 17 days. ASA treatment decreased ex vivo PGE2 production in liver homogenates and bone organ cultures of chicks, independent of the dietary lipids. Chicks fed ASA had a lower concentration of IGF-I in tibiotarsal bone compared with those not given ASA at 19 days. Serum ALPase activity was higher in chicks given M compared with those fed S, but the values were reversed with ASA feeding. This study demonstrated that both dietary fat and ASA modulated bone PGE2 biosynthesis, and that (n-3) PUFA and fat x ASA interactions altered bone morphometry.
本研究检测了膳食中（n-6）和（n-3）多不饱和脂肪酸（PUFA）以及乙酰水杨酸（ASA）对雏鸡骨灰含量、形态计量学、脂肪酸组成、体外前列腺素E2（PGE2）生物合成、组织胰岛素样生长因子-I（IGF-I）浓度以及血清碱性磷酸酶（ALPase）活性的影响。刚孵出的雏鸡饲喂含90 g/kg大豆油（S）或鲱鱼油/红花油（M）的半纯化日粮。4日龄时，将雏鸡分为四个相等的处理组，分别给予0 mg[符号：见原文]或500 mg[符号：见原文]的ASA/kg日粮：S[符号：见原文]ASA、M[符号：见原文]ASA、S[符号：见原文]ASA和M[符号：见原文]ASA。脂质和ASA处理对雏鸡的骨长度、骨灰或骨矿物质含量没有影响。与饲喂S的雏鸡相比，饲喂M的雏鸡小梁表面标记分数和组织水平骨形成率增加，且与ASA处理无关。在小梁骨体积、厚度、间距和数量方面发现了显著的脂肪×ASA交互作用效应。与饲喂M的雏鸡相比，饲喂S的雏鸡肝脏和骨骼中的20:4（n-6）浓度较高，但20:5（n-3）浓度较低。17日龄时，饲喂S的雏鸡肝脏匀浆和骨器官培养物中的体外PGE2生物合成高于饲喂M的雏鸡。ASA处理降低了雏鸡肝脏匀浆和骨器官培养物中的体外PGE2产生，与膳食脂质无关。19日龄时，饲喂ASA的雏鸡腿骨中IGF-I浓度低于未饲喂ASA的雏鸡。饲喂M的雏鸡血清ALPase活性高于饲喂S的雏鸡，但饲喂ASA后该值发生逆转。本研究表明，膳食脂肪和ASA均调节骨PGE2生物合成，且（n-3）PUFA以及脂肪×ASA相互作用改变了骨形态计量学。
