Abe H, Sugino N, Matsuda T, Kanamaru T, Oyanagi S, Mori H
Department of Anesthesiology, Kanazawa Medical University, Ishikawa.
Masui. 1996 Jan;45(1):38-43.
The effect of ulinastatin on delayed neuronal death was studied in the gerbil. The animals were divided into 2 groups according to the temperatures of the temporalis muscle, tympanic membrane, and the rectum. One group consisted of 36 animals in whom the temperature was maintained at 36.5 degrees C and the another was at 38 degrees C. They were anesthetized with isoflurane and injected intravenously with 5 ml.kg-1 of normal saline solution, 100 mg.kg-1 of alpha-tocopherol, 250,000 units.kg-1 of ulinastatin, or 500,000 units.kg-1 of ulinastatin, respectively. Transient ischemia was then induced by occluding bilateral common carotid arteries for 5 min. Six days later, they were sacrificed, and the brain tissues were fixed and stained for histopathological analysis of hippocampal CA1 regions. alpha-Tocopherol and ulinastatin prevented delayed neuronal death in the 36.5 degrees C group, but did not in the 38 degrees C group. alpha-tocopherol is recognized as an intrinsic radical scavenger, and known to prevent delayed neuronal death. Compared with 100 mg.kg-1 of alpha-tocopherol, 500,000 units.kg-1 of ulinastatin had the same protective effect on delayed neuronal death at 36.5 degrees C. We concluded that administration of ulinastatin prevented delayed neuronal death at 36.5 degrees C.
在沙鼠身上研究了乌司他丁对迟发性神经元死亡的影响。根据颞肌、鼓膜和直肠的温度将动物分为两组。一组由36只动物组成，其体温维持在36.5摄氏度，另一组为38摄氏度。用异氟烷麻醉后，分别静脉注射5ml.kg-1的生理盐水、100mg.kg-1的α-生育酚、250,000单位.kg-1的乌司他丁或500,000单位.kg-1的乌司他丁。然后通过阻断双侧颈总动脉5分钟诱导短暂性缺血。6天后，将它们处死，固定脑组织并进行染色，以对海马CA1区进行组织病理学分析。α-生育酚和乌司他丁在36.5摄氏度组中预防了迟发性神经元死亡，但在38摄氏度组中则没有。α-生育酚被认为是一种内源性自由基清除剂，已知可预防迟发性神经元死亡。与100mg.kg-1的α-生育酚相比，500,000单位.kg-1的乌司他丁在36.5摄氏度时对迟发性神经元死亡具有相同的保护作用。我们得出结论，给予乌司他丁可预防36.5摄氏度时的迟发性神经元死亡。
