Seelaender M C, Nascimento C M, Curi R, Williams J F
Dept. of Histology and Embryology, University of São Paulo, Brazil.
Biochem Mol Biol Int. 1996 Aug;39(5):1037-47. doi: 10.1080/15216549600201202.
Aspects of the lipid metabolism of Walker 256 carcinosarcoma-bearing cachectic rats (TB) were investigated during a 14 day interval of tumour growth. Food intake and body weight of the TB rats were reduced by 18% and 13%, respectively, on day 14, as compared with non-tumour-bearing animals. The tumour burden then, corresponded to 19% of total body weight. The total fat content was not different in the liver, heart, carcass, epididymal (EAT) and retroperitoneal (RPAT) adipose tissues of the two groups. The brown adipose tissue (BAT) and skeletal muscle (gastrocnemius-SM) of the TB rats had increased levels of fat (23% and 200%, respectively). Enteral absorption of 14C-triolein was decreased in the TB rats, but the liver, heart, and SM of these animals incorporated more radiolabelled lipid than the control animals, while the adipose tissues exhibited a decreased incorporation of radioactivity in relation to controls. More lipid was incorporated into the VLDL fraction secreted by the liver of TB rats, which exhibited a different distribution of the incorporated 14C-oleate in the various lipid subfractions. Ultrastructural studies showed that the hepatocytes of the TB rats had a greater incidence of lipid droplets in the cytoplasm.
在肿瘤生长的14天间隔期内，对荷Walker 256癌肉瘤恶病质大鼠（TB）的脂质代谢方面进行了研究。与无肿瘤动物相比，TB大鼠在第14天的食物摄入量和体重分别降低了18%和13%。此时肿瘤负荷相当于总体重的19%。两组动物的肝脏、心脏、胴体、附睾（EAT）和腹膜后（RPAT）脂肪组织中的总脂肪含量没有差异。TB大鼠的棕色脂肪组织（BAT）和骨骼肌（腓肠肌-SM）中的脂肪水平升高（分别为23%和200%）。TB大鼠对14C-三油酸甘油酯的肠内吸收减少，但这些动物的肝脏、心脏和SM比对照动物掺入更多的放射性标记脂质，而脂肪组织相对于对照显示出放射性掺入减少。更多的脂质掺入到TB大鼠肝脏分泌的极低密度脂蛋白（VLDL）组分中，在各种脂质亚组分中，掺入的14C-油酸呈现出不同的分布。超微结构研究表明，TB大鼠的肝细胞胞质中脂滴的发生率更高。
