对纯化痘苗病毒体外合成的病毒体相关高分子量RNA的甲基化分析。
Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.
作者信息
Nuss D L, Paoletti E
出版信息
J Virol. 1977 Jul;23(1):110-6. doi: 10.1128/JVI.23.1.110-116.1977.
Abstract
The methylation pattern of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus has been determined. Analysis of purified high-molecular-weight RNA synthesized with S-[methyl-3H]-adenosylmethionine and alpha[32P]UTP as precursors gave the following results. (i) Eessentially all molecules contained blocked and methylated structures of the type m7G(5')ppp(5')Gm and m7G(5')ppp(5')Am. (ii) There was no detectable methylation at internal sites. (iii) Under several different conditions of synthesis, the ratio of molecules containing m7G(5')ppp(5')Gm to those containing m7G(5')ppp(5')Am was imilar for both the virion-associated high-molecular-weight RNA and the virion-released 8-12S mRNA.
摘要
已确定纯化的痘苗病毒在体外合成的病毒体相关高分子量RNA的甲基化模式。以S-[甲基-³H]-腺苷甲硫氨酸和α[³²P]UTP作为前体合成的纯化高分子量RNA分析得出以下结果。(i) 基本上所有分子都含有m⁷G(5')ppp(5')Gm和m⁷G(5')ppp(5')Am类型的封闭和甲基化结构。(ii) 在内部位点未检测到甲基化。(iii) 在几种不同的合成条件下，病毒体相关高分子量RNA和病毒体释放的8 - 12S mRNA中，含有m⁷G(5')ppp(5')Gm的分子与含有m⁷G(5')ppp(5')Am的分子的比例相似。
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