痘苗病毒的可溶性核糖核酸内切酶活性：病毒粒子相关高分子量RNA的特异性切割
Soluble endoribonuclease activity from vaccinia virus: specific cleavage of virion-associated high-molecular-weight RNA.
作者信息
Paoletti E, Lipinskas B R
出版信息
J Virol. 1978 Jun;26(3):822-4. doi: 10.1128/JVI.26.3.822-824.1978.
Abstract
A soluble endoribonuclease activity was extracted from purified vaccinia virus cores by treatment with sodium-deoxycholate and dithiothreitol. The soluble enzyme readily cleaved purified virion-associated high-molecular-weight RNA to limit-sized fragments sedimenting at 8 to 12S. Purified virion-released 8 to 12S polyadenylated mRNA was not degraded by the enzyme extract. The soluble endoribonuclease did not require the presence of ribonucleoside triphosphates for activity.
摘要
通过用脱氧胆酸钠和二硫苏糖醇处理，从纯化的痘苗病毒核心中提取出一种可溶性核糖核酸内切酶活性。这种可溶性酶能轻易地将纯化的与病毒粒子相关的高分子量RNA切割成沉降系数为8至12S的有限大小片段。纯化的从病毒粒子释放的8至12S多聚腺苷酸化mRNA不会被该酶提取物降解。该可溶性核糖核酸内切酶的活性不需要核糖核苷三磷酸的存在。
相似文献
1
Soluble endoribonuclease activity from vaccinia virus: specific cleavage of virion-associated high-molecular-weight RNA.痘苗病毒的可溶性核糖核酸内切酶活性：病毒粒子相关高分子量RNA的特异性切割
J Virol. 1978 Jun;26(3):822-4. doi: 10.1128/JVI.26.3.822-824.1978.
2
Multiple roles for ATP in the synthesis and processing of mRNA by vaccinia virus: specific inhibitory effects of adenosine (beta,gamma-imido) triphosphate.ATP在痘苗病毒mRNA合成与加工中的多种作用：腺苷（β，γ-亚氨基）三磷酸的特异性抑制作用
J Virol. 1978 Aug;27(2):399-408. doi: 10.1128/JVI.27.2.399-408.1978.
3
Foot and mouth disease virus. II. Endoribonuclease activity within purified virions.
Virology. 1978 Aug;89(1):67-74. doi: 10.1016/0042-6822(78)90040-5.
4
High molecular weight virion-associated RNA of vaccinia. A possible precursor to 8 to 12 S mRNA.痘苗病毒高分子量病毒粒子相关RNA。可能是8至12S mRNA的前体。
J Biol Chem. 1977 Feb 10;252(3):872-7.
5
In vitro synthesis of a high molecular weight virion-associated RNA by vaccinia.痘苗病毒在体外合成高分子量病毒粒子相关RNA
J Biol Chem. 1977 Feb 10;252(3):866-71.
6
RNase III cleaves vesicular stomatitis virus genome-length RNAs but fails to cleave viral mRNA's.核糖核酸酶III可切割水泡性口炎病毒基因组长度的核糖核酸，但无法切割病毒信使核糖核酸。
J Virol. 1979 Apr;30(1):108-15. doi: 10.1128/JVI.30.1.108-115.1979.
7
A unique cap(m7GpppXm)-dependent influenza virion endonuclease cleaves capped RNAs to generate the primers that initiate viral RNA transcription.一种独特的依赖帽结构（m7GpppXm）的流感病毒粒子内切核酸酶切割带帽RNA，以产生启动病毒RNA转录的引物。
Cell. 1981 Mar;23(3):847-58. doi: 10.1016/0092-8674(81)90449-9.
8
Cell-free translation of purified virion-associated high-molecular-weight RNA synthesized in vitro by vaccinia virus.
J Virol. 1978 Dec;28(3):905-16. doi: 10.1128/JVI.28.3.905-916.1978.
9
Isolation and characterization of the novel polyadenylate- and polyuridylate-degrading acid endoribonuclease V from calf thymus.从小牛胸腺中分离并鉴定新型聚腺苷酸和聚尿苷酸降解酸性内切核糖核酸酶V
J Biol Chem. 1980 Jun 10;255(11):5108-12.
10
Purification and characterization of core-associated polynucleotide 5'-triphosphatase from Vaccinia virus.
J Biol Chem. 1977 May 10;252(9):3092-8.
引用本文的文献
1
Chapter 15 Poxvirus infections.第15章 痘病毒感染
Perspect Med Virol. 1985;1:599-614. doi: 10.1016/S0168-7069(08)70023-5. Epub 2008 May 29.
2
Capped and polyadenylated low-molecular-weight RNA synthesized by vaccinia virus in vitro.牛痘病毒在体外合成的加帽和聚腺苷酸化的低分子量RNA。
J Virol. 1980 Jan;33(1):208-19. doi: 10.1128/JVI.33.1.208-219.1980.
3
Analysis of vaccinia virus transcriptional complexity in vitro and in vivo: characterization of RNase T1-resistant 5'-terminal oligonucleotides.痘苗病毒在体外和体内转录复杂性的分析：核糖核酸酶T1抗性5'-末端寡核苷酸的特性研究
J Virol. 1982 May;42(2):734-41. doi: 10.1128/JVI.42.2.734-741.1982.
4
In vitro transcription of the inverted terminal repetition of the vaccinia virus genome: correspondence of initiation and cap sites.痘苗病毒基因组反向末端重复序列的体外转录：起始位点与帽位点的对应关系。
J Virol. 1981 Feb;37(2):738-47. doi: 10.1128/JVI.37.2.738-747.1981.
5
Organization and expression of the poxvirus genome.痘病毒基因组的组织与表达。
Experientia. 1982 Mar 15;38(3):285-97. doi: 10.1007/BF01949349.
6
Some enzymatic activities associated with purified parapoxvirions.一些与纯化的副痘病毒粒子相关的酶活性。
J Virol. 1983 May;46(2):384-93. doi: 10.1128/JVI.46.2.384-393.1983.
7
In vitro transcription of a cloned vaccinia virus gene by a soluble extract prepared from vaccinia virus-infected HeLa cells.用从感染痘苗病毒的HeLa细胞制备的可溶性提取物对克隆的痘苗病毒基因进行体外转录。
J Virol. 1985 Mar;53(3):822-6. doi: 10.1128/JVI.53.3.822-826.1985.
8
Vaccinia virus produces late mRNAs by discontinuous synthesis.痘苗病毒通过不连续合成产生晚期信使核糖核酸。
Cell. 1987 Jul 17;50(2):153-62. doi: 10.1016/0092-8674(87)90211-x.
9
Multiple roles for ATP in the synthesis and processing of mRNA by vaccinia virus: specific inhibitory effects of adenosine (beta,gamma-imido) triphosphate.ATP在痘苗病毒mRNA合成与加工中的多种作用：腺苷（β，γ-亚氨基）三磷酸的特异性抑制作用
J Virol. 1978 Aug;27(2):399-408. doi: 10.1128/JVI.27.2.399-408.1978.
10
Cell-free translation of purified virion-associated high-molecular-weight RNA synthesized in vitro by vaccinia virus.
J Virol. 1978 Dec;28(3):905-16. doi: 10.1128/JVI.28.3.905-916.1978.
本文引用的文献
1
Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores.痘苗病毒中的核糖核酸合成。I. 痘苗病毒核心中RNA的合成与释放机制。
J Mol Biol. 1970 May 28;50(1):1-18. doi: 10.1016/0022-2836(70)90100-2.
2
Two nucleid acid-dependent nucleoside triphosphate phosphohydrolases from vaccinia virus. Purification and characterization.来自牛痘病毒的两种核酸依赖性核苷三磷酸磷酸水解酶。纯化与特性鉴定。
J Biol Chem. 1974 May 25;249(10):3273-80.
3
An intermediate in the synthesis of poliovirus RNA.脊髓灰质炎病毒RNA合成中的一种中间体。
Proc Natl Acad Sci U S A. 1966 Aug;56(2):741-8. doi: 10.1073/pnas.56.2.741.
4
Transcriptional complexity of vaccinia virus in vivo and in vitro.痘苗病毒在体内和体外的转录复杂性。
J Virol. 1977 Sep;23(3):608-15. doi: 10.1128/JVI.23.3.608-615.1977.
5
Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.对纯化痘苗病毒体外合成的病毒体相关高分子量RNA的甲基化分析。
J Virol. 1977 Jul;23(1):110-6. doi: 10.1128/JVI.23.1.110-116.1977.
6
High molecular weight virion-associated RNA of vaccinia. A possible precursor to 8 to 12 S mRNA.痘苗病毒高分子量病毒粒子相关RNA。可能是8至12S mRNA的前体。
J Biol Chem. 1977 Feb 10;252(3):872-7.
7
In vitro synthesis of a high molecular weight virion-associated RNA by vaccinia.痘苗病毒在体外合成高分子量病毒粒子相关RNA
J Biol Chem. 1977 Feb 10;252(3):866-71.
Zotero 插件