水泡性口炎病毒在体外合成的甲基化信使核糖核酸的5'末端结构
The 5' terminal structure of the methylated mRNA synthesized in vitro by vesicular stomatitis virus.
作者信息
Abraham G, Rhodes D P, Banerjee A K
出版信息
Cell. 1975 May;5(1):51-8. doi: 10.1016/0092-8674(75)90091-4.
PMID:165900
Abstract
The 5' terminal structure of the mRNA synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus in the presence of S-adenosyl-L-methione consists of 7-methyl guanosine linked to 2'-O-methyl adenosine through a 5'-5' pyrophosphate bond as m7G(5')ppp(5')A-m-p ... The alpha and beta phosphated of GTP and alpha phosphate of ATP are incorporated into the blocked 5' terminal structure.
摘要
在存在S-腺苷-L-甲硫氨酸的情况下，由水泡性口炎病毒的病毒体相关RNA聚合酶体外合成的mRNA的5'末端结构由通过5'-5'焦磷酸键与2'-O-甲基腺苷相连的7-甲基鸟苷组成，即m7G(5')ppp(5')A-m-p... GTP的α和β磷酸以及ATP的α磷酸被掺入到封闭的5'末端结构中。
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