从患病斑节对虾（Penaeus monodon）中分离出的溶藻弧菌的毒力。
Virulence of Vibrio alginolyticus isolated from diseased tiger prawn, Penaeus monodon.
作者信息
Lee K K, Yu S R, Chen F R, Yang T I, Liu P C
机构信息
Department of Aquaculture, National Taiwan Ocean University, Keelug, ROC.
出版信息
Curr Microbiol. 1996 Apr;32(4):229-31. doi: 10.1007/s002849900041.
Outbreaks of mass mortality among cultured tiger prawns (Penaeus monodon) with white spotted syndrome (WSS) in the carapace occurred in the summer of 1994 in I-Lan, Taiwan. A swarming strain Val was isolated from hemolymph of the moribund prawns with tryptic soy agar (TSA, supplemented with 1% NaCl, Oxoid) and/or thiosulfate citrate bile salt sucrose (TCBS, Difco) agar. This strain was characterized and identified to be Vibrio alginolyticus. The strain was susceptible to antibiotics such as chloramphenicol, ciprofloxacin, doxycycline hydrochloride, nalidixic acid, oxolic acid, and oxytetracycline while resistant to ampicillin, novobiocin, penicillin G, sulfisoxazole, and sulfonamide. The bacteria and their extracellular products (ECP) were lethal to both tiger prawns (P. monodon) and kuruma prawns (P. japonicus) with LD50 values of 1.13 x 10(5), 2.46 x 10(5) CFU/g, and 0.23, 0.63 micrograms protein/g prawn body weight, respectively.
1994年夏天，台湾宜兰养殖的斑节对虾（Penaeus monodon）出现了大规模死亡疫情，虾壳出现白斑综合征（WSS）。从濒死对虾的血淋巴中，使用胰蛋白胨大豆琼脂（TSA，添加1% NaCl，Oxoid公司生产）和/或硫代硫酸盐柠檬酸盐胆盐蔗糖（TCBS，Difco公司生产）琼脂分离出一种群游菌株Val。该菌株经鉴定为溶藻弧菌。该菌株对氯霉素、环丙沙星、盐酸多西环素、萘啶酸、草酸和土霉素等抗生素敏感，而对氨苄青霉素、新生霉素、青霉素G、磺胺异恶唑和磺胺类药物耐药。该细菌及其胞外产物（ECP）对斑节对虾（P. monodon）和日本对虾（P. japonicus）均具有致死性，LD50值分别为1.13 x 10(5)、2.46 x 10(5) CFU/g以及0.23、0.63微克蛋白质/克虾体重。
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