Toth D P, Guenther L C, Shum D T
Division of Dermatology, Victoria Hospital, University of Western Ontario, London, Canada.
J Dermatol Sci. 1996 Jan;11(1):36-40. doi: 10.1016/0923-1811(95)00417-3.
Proliferating cell nuclear antigen (PCNA) is a nuclear protein which is correlated with the S-phase of the cell cycle and has been utilized by many investigators as a marker of cell proliferation. A previous immunohistochemical study revealed increased PCNA staining in clinically and histologically aggressive basal cell carcinoma and the present study evaluated the prognostic value of PCNA in clinical recurrence of primary basal cell carcinoma. Thirty patients with primary basal cell carcinoma (BCC) treated with shave biopsy followed by electrodesiccation in 1989 and have been regularly followed up for local recurrence were selected for this study. The histology of their primary BCC's was reviewed and the presence of PCNA in the tumor cells was studied immunohistochemically. Ninety-six percent of the non-recurrent BCC's had < 10% of tumor cells showing a positive staining for PCNA whereas 100% of the BCC's that recurred showed more than 30% of tumor cells staining positive for PCNA. In comparison, 88.9% of the non-recurring group showed non-aggressive histological features and only 66.7% of the recurring group was aggressive by microscopic appearance. In summary, the PCNA staining appeared to be superior to traditional histologic features in predicting clinical recurrence in primary BCC's and further prospective studies in a larger patient group are warranted.
增殖细胞核抗原（PCNA）是一种核蛋白，与细胞周期的S期相关，许多研究人员将其用作细胞增殖的标志物。先前的一项免疫组织化学研究显示，在临床和组织学上侵袭性的基底细胞癌中PCNA染色增加，本研究评估了PCNA在原发性基底细胞癌临床复发中的预后价值。本研究选取了30例1989年接受剃除活检继以电干燥治疗的原发性基底细胞癌（BCC）患者，这些患者一直在定期随访局部复发情况。回顾了他们原发性BCC的组织学，并通过免疫组织化学研究了肿瘤细胞中PCNA的存在情况。96%的未复发BCC中，<10%的肿瘤细胞PCNA染色呈阳性，而100%复发的BCC中，超过30%的肿瘤细胞PCNA染色呈阳性。相比之下，88.9%的未复发组表现出非侵袭性组织学特征，而复发组中只有66.7%在显微镜下表现为侵袭性。总之，在预测原发性BCC的临床复发方面，PCNA染色似乎优于传统组织学特征，有必要在更大的患者群体中进行进一步的前瞻性研究。
