Yoshida K, Fujimura M, Yanai N, Fujita Y
Department of Biotechnology, Fukuyama University, Hiroshima, Japan.
DNA Res. 1995 Dec 31;2(6):295-301. doi: 10.1093/dnares/2.6.295.
Within the framework of an international project for the sequencing of the entire Bacillus subtilis genome, a 23-kb chromosomal segment, which covers the region between the iol and hut operons, has been cloned and sequenced, creating a 99-kb contig from the gnt operon to the wapA locus. This region (23351 bp) contains 25 complete open reading frames (ORFs; genes) including deoR, dra, nupC and pdp and two partial ones. The region (5140 bp) containing these four genes, being also sequenced by H. H. Saxild et al., was sequenced by subjecting a long polymerase chain reaction product to random sequencing using phage M13mp19. However, we could detect no conflict, between two independently determined sequences, which could be attributed to our sequencing method. A homology search for the 24 newly identified gene products revealed significant homology to known proteins in 14 of them. It was notable that three proteins, encoded by the successive genes (yxeMNO), exhibited meaningful homology to the E. coli GlnHPQ products constituting a periplasmic ATP-dependent transport system for glutamine.
在一个对整个枯草芽孢杆菌基因组进行测序的国际项目框架内，一个覆盖iol和hut操纵子之间区域的23 kb染色体片段已被克隆和测序，形成了一个从gnt操纵子到wapA位点的99 kb重叠群。该区域（23351 bp）包含25个完整的开放阅读框（ORF；基因），包括deoR、dra、nupC和pdp以及两个部分开放阅读框。H. H. Saxild等人也对包含这四个基因的区域（5140 bp）进行了测序，我们是通过对长聚合酶链反应产物进行随机测序，并使用噬菌体M13mp19来对该区域进行测序的。然而，我们在两个独立测定的序列之间未检测到任何冲突，这可能归因于我们的测序方法。对24个新鉴定的基因产物进行同源性搜索发现，其中14个与已知蛋白质具有显著同源性。值得注意的是，由连续基因（yxeMNO）编码的三种蛋白质与大肠杆菌GlnHPQ产物表现出有意义的同源性，后者构成了一种用于谷氨酰胺的周质ATP依赖性转运系统。
