Yoshida K, Shindo K, Sano H, Seki S, Fujimura M, Yanai N, Miwa Y, Fujita Y
Department of Biotechnology, Faculty of Engineering, Fukuyama University, Hiroshima, Japan.
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3113-23. doi: 10.1099/13500872-142-11-3113.
Within the framework of an international project for the sequencing of the entire Bacillus subtilis genome, this paper communicates the sequencing of a chromosome region containing the lic and cel loci (65 kb), which creates a 177 kb contig covering the region from gnt to sacXY. This 65 kb region contains 64 ORFs (62 complete and two partial genes). The 14th, 15th and 17th genes correspond to licT, licS and katE, encoding the antiterminator for licS transcription, beta-glucanase (lichenase) and catalase 2, respectively. The 11th, 30th, 36th, 39th, 41st, 45th-48th, 51st and 58th genes are designated deaD, pepT, galE, aldY, msmX, cydABCD, sigY and katX because their products probably encode ATP-dependent RNA helicase, tripeptidase, UDP-glucose 4-epimerase, aldehyde dehydrogenase, multiple sugar-binding transport ATP-binding protein, the respective components of cytochrome d ubiquinol oxidase and ATP-binding cassette transporter, sigma-factor of RNA polymerase and catalase, respectively. The 60th-64th genes are celRABCD, which are probably involved in cellobiose utilization. Gene organization and gene features in the gnt-sacXY region are discussed.
在一项对整个枯草芽孢杆菌基因组进行测序的国际项目框架内，本文报道了一个包含 lic 和 cel 基因座（65 kb）的染色体区域的测序结果，该区域形成了一个 177 kb 的重叠群，覆盖了从 gnt 到 sacXY 的区域。这个 65 kb 的区域包含 64 个开放阅读框（62 个完整基因和 2 个部分基因）。第 14、15 和 17 个基因分别对应 licT、licS 和 katE，它们分别编码 licS 转录的抗终止子、β-葡聚糖酶（地衣酶）和过氧化氢酶 2。第 11、30、36、39、41、45 - 48、51 和 58 个基因分别被命名为 deaD、pepT、galE、aldY、msmX、cydABCD、sigY 和 katX，因为它们的产物可能分别编码依赖 ATP 的 RNA 解旋酶、三肽酶、UDP-葡萄糖 4-表异构酶、醛脱氢酶、多种糖结合转运 ATP 结合蛋白、细胞色素 d 泛醇氧化酶的各个组分和 ATP 结合盒转运体、RNA 聚合酶的 sigma 因子以及过氧化氢酶。第 60 - 64 个基因是 celRABCD，它们可能参与纤维二糖的利用。文中讨论了 gnt - sacXY 区域的基因组织和基因特征。
