Yoshida K, Seki S, Fujimura M, Miwa Y, Fujita Y
Department of Biotechnology, Faculty of Engineering, Fukuyama University, Hiroshima, Japan.
DNA Res. 1995;2(2):61-9. doi: 10.1093/dnares/2.2.61.
Within the framework of an international project for the sequencing of the entire Bacillus subtilis genome, a 36-kb chromosome segment, which covers the region between the gnt and iol operons, has been cloned and sequenced. This region (36447 bp) contains 33 complete open reading frames (ORFs; genes) including the four gnt genes and one partial gene. A homology search for the products of the 33 complete ORFs revealed significant homology to known proteins in 16 of them such as tetracycline resistance protein (Clostridium perfringens), asparagine synthetase (Arabidopsis thaliana), aldehyde dehydrogenase (Pseudomonas oleovorans), 2,5-dichloro-2,5-cyclohexadiene-1, 4-diol dehydrogenase (P. paucimobilis), heat shock protein HtpG (Escherichia coli), galactose-proton symporter (E. coli), auxin-induced protein (common tobacco), glucitol operon repressor (E. coli) and methylmalonate-semialdehyde dehydrogenase (P. aeruginosa). Unlike the regions we sequenced so far, this region contained two short sequence multiplications: one was a tandem sequence duplication (409 and 410 bp), and the other a triplication consisting of two highly conserved 118-bp tandem sequences preceded by a less conserved similar sequence (129 bp). The reasons for the presence of these sequence multiplications in the gnt to iol region were deduced.
在一个对枯草芽孢杆菌全基因组进行测序的国际项目框架内，一个覆盖gnt和iol操纵子之间区域的36 kb染色体片段已被克隆并测序。该区域（36447 bp）包含33个完整的开放阅读框（ORF；基因），其中包括4个gnt基因和1个部分基因。对这33个完整ORF的产物进行同源性搜索发现，其中16个与已知蛋白质具有显著同源性，如四环素抗性蛋白（产气荚膜梭菌）、天冬酰胺合成酶（拟南芥）、醛脱氢酶（食油假单胞菌）、2,5-二氯-2,5-环己二烯-1,4-二醇脱氢酶（少动假单胞菌）、热休克蛋白HtpG（大肠杆菌）、半乳糖-质子同向转运体（大肠杆菌）、生长素诱导蛋白（普通烟草）、葡萄糖醇操纵子阻遏物（大肠杆菌）和甲基丙二酸半醛脱氢酶（铜绿假单胞菌）。与我们迄今测序的区域不同，该区域包含两个短序列重复：一个是串联序列重复（409和410 bp），另一个是由两个高度保守的118 bp串联序列组成的三倍体，其前面是一个保守性较低的相似序列（129 bp）。推测了gnt至iol区域中这些序列重复存在的原因。
