Seif-El-Nasr M, El-Fattah A A
Department of Pharmacology, Cairo University, Egypt.
Pharmacol Res. 1995 Nov;32(5):273-8. doi: 10.1016/s1043-6618(05)80014-3.
The influence of ginkgo biloba extract on the lipid peroxide product (malondialdehyde, MDA), glutathione (GSH) and phospholipids levels as well as superoxide dismutase (SOD, 1.15.1.1) and lactate dehydrogenase (LDH, 1.1.1.27) activities in rat brain after occlusion of common carotid arteries was investigated. Two experimental models were studied: 60 min ischaemia without reperfusion and 60 min ischaemia followed by 60 min reperfusion. Compared to sham-operated animals, ischaemia followed by reperfusion increased cytosolic LDH activity and mitochondrial lipid peroxide content and decreased the superoxide dismutase activity and mitochondrial total phospholipids level. Preischaemic administration of ginkgo biloba extract (150 mg kg-1, p.o.) could normalize the SOD activity of the rat brain. The extract was also able to reduce the lipid peroxide and phospholipids contents of the mitochondrial rat brain. These effects could be explained on the basis of the antioxidant property of ginkgo biloba extract and suggests its beneficial role in the protection against post-ischaemic injury.
研究了银杏叶提取物对大鼠双侧颈总动脉闭塞后脑组织脂质过氧化物产物（丙二醛，MDA）、谷胱甘肽（GSH）和磷脂水平以及超氧化物歧化酶（SOD，1.15.1.1）和乳酸脱氢酶（LDH，1.1.1.27）活性的影响。研究了两种实验模型：60分钟缺血无再灌注和60分钟缺血后再灌注60分钟。与假手术动物相比，缺血后再灌注增加了胞质LDH活性和线粒体脂质过氧化物含量，降低了超氧化物歧化酶活性和线粒体总磷脂水平。缺血前给予银杏叶提取物（150mg/kg，口服）可使大鼠脑SOD活性恢复正常。该提取物还能够降低大鼠脑线粒体的脂质过氧化物和磷脂含量。这些作用可以基于银杏叶提取物的抗氧化特性来解释，并表明其在预防缺血后损伤中的有益作用。
