Binnie C, Liao L, Walczyk E, Malek L T
Cangene Corporation, Mississauga, ON, Canada.
Can J Microbiol. 1996 Mar;42(3):284-8. doi: 10.1139/m96-041.
A gene encoding a Streptomyces lividans homologue of the chymotrypsin-like serine protease (SAM-P20) of Streptomyces albogriseolus was isolated using the Streptomyces griseus prtB gene as a hybridization probe. Nucleotide sequence analysis of a representative clone uncovered the possible presence of a sequence of 900 nucleotides encoding 300 amino acids, including a putative "prepro" region of 115 amino acids. Alignment of the predicted amino acid sequence of this putative gene with other members of the family of Streptomyces extracellular chymotrypsin-like proteases indicated a high degree of homology in all cases, especially with the SAM-P20 protease. This gene product has been identified as the second member of a potentially larger family of SAL (SAM-P20-like) proteases in S. lividans 66.
以灰色链霉菌prtB基因作为杂交探针，分离出了编码白灰链霉菌类胰凝乳蛋白酶样丝氨酸蛋白酶（SAM-P20）的天蓝色链霉菌同源基因。对一个代表性克隆进行核苷酸序列分析发现，可能存在一个900个核苷酸的序列，编码300个氨基酸，其中包括一个115个氨基酸的假定“前原”区域。将该假定基因的预测氨基酸序列与链霉菌细胞外类胰凝乳蛋白酶家族的其他成员进行比对，结果表明在所有情况下都具有高度同源性，尤其是与SAM-P20蛋白酶。该基因产物已被鉴定为天蓝色链霉菌66中可能更大的SAL（SAM-P20样）蛋白酶家族的第二个成员。
