Taguchi S, Ogawa T, Endo T, Momose H
Department of Biological Science and Technology, Science University of Tokyo, Japan.
Biosci Biotechnol Biochem. 1997 May;61(5):909-13. doi: 10.1271/bbb.61.909.
A gene encoding a homolog of the Streptomyces chymotrypsin-like serine protease, SAM-P20, was identified downstream of the sam-p20 gene and designated SAM-P20D. This gene has two tandem Shine Dalgarno sequences and two initiation codons. We have established vector systems with the function of tyrosinase gene-bone melanin pigmentation as a reporter for sam-p20D gene expression in Streptomyces coelicolor in order to identify the promoter and terminator activities. Using this system, the sam-p20D gene was suggested to be transcribed monocistronically.
在sam-p20基因下游鉴定出一个编码类胰凝乳蛋白酶链霉菌丝氨酸蛋白酶同系物的基因SAM-P20，并将其命名为SAM-P20D。该基因有两个串联的Shine Dalgarno序列和两个起始密码子。为了鉴定启动子和终止子活性，我们建立了以酪氨酸酶基因-骨黑色素沉着功能作为天蓝色链霉菌中sam-p20D基因表达报告基因的载体系统。利用该系统，提示sam-p20D基因以单顺反子形式转录。
