Taguchi S, Endo T, Naoi Y, Momose H
Department of Biological Science and Technology, Science University of Tokyo, Chiba, Japan.
Biosci Biotechnol Biochem. 1995 Jul;59(7):1386-8. doi: 10.1271/bbb.59.1386.
A gene encoding a homolog of the chymotrypsin-like serine protease (SAM-P20), which was isolated as the target enzyme of a protease inhibitor (SSI), was cloned from Streptomyces lividans 66. This gene contained an open reading frame of 1065 nucleotides encoding 354 amino acid residues with a putative prepro portion of 157 amino acid residues. The deduced amino acid sequence of the cloned gene had significant homology to those of members of Streptomyces extracellular chymotrypsin-like protease family. By Southern blot analysis, it was suggested that protease genes of this type are found at a high frequency in Streptomyces. In this sense, we propose to categorize this protease as a member of the 'SAL' series (SAM-P20-like proteases).
从淡紫灰链霉菌66中克隆出一个编码类胰凝乳蛋白酶丝氨酸蛋白酶（SAM-P20）同源物的基因，该基因是作为一种蛋白酶抑制剂（SSI）的靶酶而分离得到的。该基因包含一个1065个核苷酸的开放阅读框，编码354个氨基酸残基，推测其前原区有157个氨基酸残基。克隆基因推导的氨基酸序列与链霉菌细胞外类胰凝乳蛋白酶家族成员的序列具有显著同源性。通过Southern杂交分析表明，这种类型的蛋白酶基因在链霉菌中高频存在。从这个意义上讲，我们建议将这种蛋白酶归类为“SAL”系列（类SAM-P20蛋白酶）的成员。
