Smiałowska M, Bijak M, Sopala M, Tokarski K
Department of Neurobiology, Polish Academy of Sciences, Kraków.
Neuropeptides. 1996 Feb;30(1):7-12. doi: 10.1016/s0143-4179(96)90048-7.
The effect of neuropeptide Y (NPY) on the picrotoxin-induced activity was studied in rat brain hippocampal slices in vitro and after intrahippocampal injection in vivo. In the hippocampal slices, NPY (0.1-0.5 microM) inhibited the picrotoxin-induced epileptiform activity recorded extracellularly in CA1 and CA3 hippocampal pyramidal cells. Similar inhibition was induced by the Y2 receptor agonist NPY13-36, which indicates that the effect of NPY was due to activation of Y2 receptors. In behavioural studies, rats with chronically implanted cannulae were injected unilaterally into the CA1 hippocampal region with a 1 ml volume of the studied substances. Picrotoxin in a dose of 1 mg (1.6 nmol) induced behavioural excitation, shakes and weak signs of epileptic behaviour. NPY in a dose of 2 mg (470 pmol), but not 1 mg, inhibited some excitatory effects of picrotoxin, but did not change the epileptic symptoms. The obtained results suggest that NPY has an inhibitory action in the hippocampus, which can be observed in vitro and also in a behavioural study.
在体外大鼠脑海马切片以及海马内注射后在体内研究了神经肽Y（NPY）对印防己毒素诱导活性的影响。在海马切片中，NPY（0.1 - 0.5微摩尔）抑制了在海马CA1和CA3锥体神经元细胞外记录到的印防己毒素诱导的癫痫样活动。Y2受体激动剂NPY13 - 36也诱导了类似的抑制作用，这表明NPY的作用是由于Y2受体的激活。在行为学研究中，给长期植入套管的大鼠单侧海马CA1区注射1毫升研究物质。1毫克（1.6纳摩尔）剂量的印防己毒素诱导行为兴奋、震颤以及癫痫行为的轻微迹象。2毫克（470皮摩尔）剂量的NPY，但1毫克剂量的NPY没有，抑制了印防己毒素的一些兴奋作用，但没有改变癫痫症状。所得结果表明，NPY在海马中具有抑制作用，这在体外以及行为学研究中均能观察到。
