Smiałowska M, Sopala M, Tokarski K
Department of Neurobiology, Polish Academy of Sciences, Kraków, Poland.
Neuropeptides. 1996 Feb;30(1):67-71. doi: 10.1016/s0143-4179(96)90057-8.
The aim of the present study was to investigate the influence of intrahippocampal neuropeptide Y (NPY) administration on the rats' behaviour. The CA1 field or dentate area (GD) of the dorsal hippocampus was chronically implanted with intracerebral cannulae. NPY (or redistilled water in the control group) was injected bilaterally or unilaterally in a volume of 1 microliter to each hippocampus, in a dose of 1 or 2 micrograms per rat. A separate group of rats was pretreated with amphetamine (1 mg/kg, s.c.), 15 min before a bilateral microinjection of NPY (2 micrograms) into the CA1 region. Immediately after the intrahippocampal injection, the rats' behaviour was tested in an open field box. It was found that NPY did not change the locomotor and the exploratory activity after either CA1 or GD administration to non-pretreated animals. In amphetamine pretreated rats, NPY injected into the CA1 field inhibited the amphetamine induced increase in sniffing and rearing and, to a lesser extent, the number of line-crossings. The obtained results may suggest an inhibitory action of NPY in the CA1 hippocampal field on the behavioural hyperactivity.
本研究的目的是探讨海马内注射神经肽Y（NPY）对大鼠行为的影响。将脑内套管长期植入背侧海马的CA1区或齿状区（GD）。以每只大鼠1或2微克的剂量，向每个海马双侧或单侧注射1微升NPY（对照组注射重蒸馏水）。另一组大鼠在双侧向CA1区微量注射NPY（2微克）前15分钟，皮下注射苯丙胺（1毫克/千克）进行预处理。海马内注射后，立即在旷场箱中测试大鼠的行为。结果发现，对未预处理的动物，向CA1区或GD区注射NPY后，其运动和探索活动没有改变。在苯丙胺预处理的大鼠中，向CA1区注射NPY可抑制苯丙胺诱导的嗅探和竖毛增加，以及在较小程度上抑制穿越线条的次数。所得结果可能提示海马CA1区的NPY对行为多动有抑制作用。
