Green S J, Wilson J F
Department of Pharmacology and Therapeutics, University of Wales College of Medicine, Cardiff, U.K.
J Anal Toxicol. 1996 Mar-Apr;20(2):121-3. doi: 10.1093/jat/20.2.121.
Six groups of six male, hooded, Lister rats were administered methadone in their drinking water over the concentration range 0-0.25 mg/mL for 6 weeks. Black pigmented and white nonpigmented hair and trunk blood samples were collected. Plasma and alkali digests of hair were analyzed for methadone by radioimmunoassay. Hair melanin content was estimated in digests by turbidimetry. Oral methadone intake rose in a linear fashion over the six dose groups, and plasma methadone concentration followed a similar trend. The methadone content of both white and black hair increased over the six dose groups, but concentrations were significantly higher in black compared with white hair. The mean ratio of methadone concentration between black and white hair was 21.3:1, and the mean ratio in hair melanin content was 3.5:1. The results demonstrate that pigmented hair incorporates larger quantities of methadone than nonpigmented hair and that methadone binds with higher affinity to the pigmented components as compared with other components of hair.
将六组每组六只雄性带帽利斯特大鼠置于含美沙酮的饮用水中，美沙酮浓度范围为0 - 0.25毫克/毫升，持续6周。采集黑色有色素毛发、白色无色素毛发及躯干血样。通过放射免疫分析法分析毛发的血浆和碱消化液中的美沙酮。通过比浊法估算消化液中的毛发黑色素含量。在六个剂量组中，口服美沙酮摄入量呈线性上升，血浆美沙酮浓度也呈现类似趋势。在六个剂量组中，白色和黑色毛发中的美沙酮含量均增加，但黑色毛发中的浓度显著高于白色毛发。黑色与白色毛发中美沙酮浓度的平均比值为21.3:1，毛发黑色素含量的平均比值为3.5:1。结果表明，有色素毛发比无色素毛发摄取的美沙酮量更大，并且与毛发的其他成分相比，美沙酮与有色素成分的结合亲和力更高。
