Ranaldi F, Vanni P, Giachetti E
Département des Sciences Biochimiques, Université de Florence, Italie.
C R Seances Soc Biol Fil. 1996;190(2-3):299-309.
In this work on Rhodotorula gracilis, we studied the level of the representative enzymes of foundamental metabolic ways (HK, PK, G6PDH and IDH) and those of ICL and MS key enzymes of glyoxylate cycle with different carbone sources. The glucose appears the best source of carbone for all the tested enzymes except ICL and MS which are practically absent in the cells developed with this carbone source. In presence of acetate and ethanol we have obtained the expression of the glyoxylate cycle enzymes (ICL and MS). Moreover we obtained the operativity of the glyoxylate cycle in the lipid-rich cells or cells developped on neutral detergents or anionics similar to the fatty acids.
在这项关于纤细红酵母的研究中,我们研究了不同碳源条件下基础代谢途径的代表性酶(己糖激酶、丙酮酸激酶、葡萄糖-6-磷酸脱氢酶和异柠檬酸脱氢酶)以及乙醛酸循环关键酶异柠檬酸裂解酶和苹果酸合酶的水平。葡萄糖似乎是所有测试酶的最佳碳源,但异柠檬酸裂解酶和苹果酸合酶除外,在用这种碳源培养的细胞中几乎不存在这两种酶。在乙酸盐和乙醇存在的情况下,我们检测到了乙醛酸循环酶(异柠檬酸裂解酶和苹果酸合酶)的表达。此外,我们还发现富含脂质的细胞或在中性洗涤剂或类似脂肪酸的阴离子表面活性剂上生长的细胞中,乙醛酸循环具有活性。