Nikolovski S, Faulkner D L, Palmer T N, Fournier P A
Department of Biochemistry, University of Western Australia, Nedlands, Australia.
Acta Physiol Scand. 1996 Aug;157(4):427-34. doi: 10.1046/j.1365-201X.1996.507273000.x.
During recovery from high intensity exercise, substantial and rapid muscle glycogen repletion from endogenous carbon sources is reported in a variety of vertebrate species, the rat being the only reported exception. The major aim of this study was to re-examine the process of glycogen repletion during recovery from high intensity exercise in the rat. In response to 3 min of vigorous swimming, muscle glycogen concentrations decrease markedly from initial levels of 20.2 +/- 1.5 and 21.2 +/- 0.9 mumol g-1 to 6.4 +/- 1.1 and 7.9 +/- 1.4 mumol g-1 in the tibialis anterior and plantaris muscles respectively. The equivalent of 58% of the glycogen carbons mobilized during exercise by the plantaris and 73% of that mobilized by the tibialis anterior muscle is repleted within 1 h following exercise. Using the hepatectomized rat as experimental model, a secondary aim of the study was to evaluate whether the liver is essential for the repletion of muscle glycogen. Although the absence of significant differences in the magnitude of post-exercise muscle glycogen repletion between sham-operated and hepatectomized rats suggests that the resynthesis of muscle glycogen can take place in the absence of hepatic gluconeogenesis, the present study identifies several limitations in the use of acute hepatectomy. Overall, the present study indicates that, in contrast to published views, the rat resembles other vertebrates in that it can support extensive muscle glycogen repletion from endogenous carbon sources during the recovery phase following high intensity exercise.
在从高强度运动恢复过程中，据报道多种脊椎动物能从内源性碳源大量且快速地补充肌肉糖原，大鼠是唯一报道的例外。本研究的主要目的是重新审视大鼠在从高强度运动恢复过程中的糖原补充过程。在3分钟剧烈游泳后，胫骨前肌和跖肌的肌肉糖原浓度分别从初始水平20.2±1.5和21.2±0.9μmol g-1显著降至6.4±1.1和7.9±1.4μmol g-1。运动后1小时内，跖肌运动期间动员的糖原碳的58%以及胫骨前肌动员的糖原碳的73%得到补充。以肝切除大鼠作为实验模型，本研究的第二个目的是评估肝脏对于肌肉糖原补充是否必不可少。尽管假手术组和肝切除组大鼠运动后肌肉糖原补充量没有显著差异，这表明在没有肝脏糖异生的情况下肌肉糖原的再合成也能发生，但本研究指出了急性肝切除应用中的一些局限性。总体而言，本研究表明，与已发表的观点相反，大鼠与其他脊椎动物相似，即在高强度运动后的恢复阶段，它能够从内源性碳源支持广泛的肌肉糖原补充。
