Soleo L, Defazio G, Scarselli R, Zefferino R, Livrea P, Foà V
Istituto di Medicina del Lavoro, Università di Bari, Policlinico, Italy.
Arch Toxicol. 1996;70(10):678-82. doi: 10.1007/s002040050328.
Agricultural exposure to the organomanganese fungicide MANEB (manganese-ethylene-bis-dithiocarbamate) may induce an extrapyramidal syndrome resembling parkinsonism. To evaluate the relative role of manganese (Mn) and ethylene-bis-dithiocarbamate (EBDTC) in the hazard of organomanganese fungicides, we studied the effects of MANCOZEB (Mn-Zinc-EBDTC) and ZINEB (Zinc-EBDTC) on serumless dissociated mesencephalic-striatal primary coculture. High affinity 3H-dopamine (DA) and 14C GABA uptakes as well as immunocytochemical staining of tyrosine hydroxylase (TH)-containing cells were used as specific functional markers of DA and GABA neuron viability. Both MANCOZEB and ZINEB, at 10 and 50 microM concentrations, dose dependently reduced DA and GABA viability parameters. These data suggest that EBDTC rather than Mn may be primarily responsible for the cytotoxicity of organomanganese fungicides on neuronal systems relevant to the pathophysiology of parkinsonism.
农业接触有机锰杀菌剂代森锰锌（锰-亚乙基-双-二硫代氨基甲酸盐）可能会引发一种类似帕金森症的锥体外系综合征。为评估锰（Mn）和亚乙基-双-二硫代氨基甲酸盐（EBDTC）在有机锰杀菌剂危害中的相对作用，我们研究了代森锰锌（锰-锌-EBDTC）和代森锌（锌-EBDTC）对无血清解离中脑-纹状体原代共培养物的影响。高亲和力3H-多巴胺（DA）和14C-γ-氨基丁酸（GABA）摄取以及含酪氨酸羟化酶（TH）细胞的免疫细胞化学染色被用作DA和GABA神经元活力的特异性功能标志物。代森锰锌和代森锌在10和50微摩尔浓度下，均剂量依赖性地降低了DA和GABA活力参数。这些数据表明，EBDTC而非Mn可能是有机锰杀菌剂对与帕金森症病理生理学相关的神经元系统产生细胞毒性的主要原因。
