Knöchel W, Asahi K, Born J, Tiedemann H
Mol Biol Rep. 1977 Jun;3(4):269-74. doi: 10.1007/BF00368296.
Chicken embryos were pulse-labelled in vivo with [3H]uridine (10 min), the chromatin isolated and treated with DNAse I. The residual chromatin was separated from the degradation products by centrifugation. The nascent pulse-labelled RNA is completely recovered in the residual chromatin even after prolonged incubation with DNAase I, whereas the DNA is completely degraded to 80 base polynucleotide fragments and smaller fragments.
用[3H]尿苷对鸡胚进行体内脉冲标记（10分钟），分离染色质并用DNA酶I处理。通过离心将残留的染色质与降解产物分离。即使在与DNA酶I长时间孵育后，新生的脉冲标记RNA仍能在残留的染色质中完全回收，而DNA则完全降解为80个碱基的多核苷酸片段和更小的片段。
