Albrecht J, Hemminki K
Biochim Biophys Acta. 1977 Mar 2;475(1):152-9. doi: 10.1016/0005-2787(77)90349-5.
Chromatins of four embryonic chick tissues were digested with DNAase II and the MgCl2-soluble and insoluble chromatin fractions were isolated. The MgCl2-soluble fractions displayed a high protein/DNA ratio and enrichment of nascent RNA as compared to the MgCl2-insoluble fractions. The recovery of DNA and protein in the MgC12-soluble chromatin of erythrocytes was much lower than that in the other cell types. The difference is likely to be associated with the low transcriptional activity of the erythrocyte chromatin. In polyacrylamide gels histones appeared to be the predominant protein constituent of the MgCl2-insoluble chromatins. Brain, skin and muscle displayed an apparently similar group of actively labelled nonhistones at 70 000 to 100 000 daltons. These nonhistones were not observed in erythrocytes. The MgCl2-soluble chromatins of erythrocytes, brain, skin and muscle had a prominent group of polypeptides at approx. 40 000 daltons. In all tissues except for erythrocytes the group of proteins was actively labelled. These polypeptides are suggested to be the major structural protein constituent of the MgCl-soluble chromatin.
用脱氧核糖核酸酶II消化四种鸡胚组织的染色质，并分离出氯化镁可溶性和不溶性染色质组分。与氯化镁不溶性组分相比，氯化镁可溶性组分显示出高蛋白/DNA比率和新生RNA的富集。红细胞的氯化镁可溶性染色质中DNA和蛋白质的回收率远低于其他细胞类型。这种差异可能与红细胞染色质的低转录活性有关。在聚丙烯酰胺凝胶中，组蛋白似乎是氯化镁不溶性染色质的主要蛋白质成分。脑、皮肤和肌肉在70000至100000道尔顿处显示出一组明显相似的活跃标记的非组蛋白。在红细胞中未观察到这些非组蛋白。红细胞、脑、皮肤和肌肉的氯化镁可溶性染色质在约40000道尔顿处有一组突出的多肽。除红细胞外，所有组织中的这组蛋白质都被活跃标记。这些多肽被认为是氯化镁可溶性染色质的主要结构蛋白成分。
