Borman R A, Burleigh D E
Department of Pharmacology, Queen Mary and Westfield College, London, UK.
Eur J Pharmacol. 1996 Aug 15;309(3):271-4. doi: 10.1016/0014-2999(96)00466-9.
The aim of this study was to characterise the 5-HT receptor(s) mediating secretory responses of isolated human colonic mucosa to 5-HT. Sheets of muscle-stripped mucosa from proximal (ascending) and distal (sigmoid) human colon were set up in Ussing chambers for measurement of short-circuit current (Isc). Serosal application of 5-HT led to non-neural, concentration-dependent increases in Isc. Desensitisation to 5-HT was observed with ascending and sigmoid colonic mucosa. Using selective 5-HT antagonists we have shown that 5-HT induces secretion in sigmoid colon via a 5-HT2A receptor. In ascending colon a combination of 5-HT2A and 5-HT4 receptors appears to be involved.
本研究的目的是鉴定介导5-羟色胺（5-HT）对离体人结肠黏膜分泌反应的5-HT受体。将来自人近端（升结肠）和远端（乙状结肠）结肠的无肌层黏膜片置于Ussing小室中，以测量短路电流（Isc）。5-HT的浆膜应用导致Isc出现非神经性、浓度依赖性增加。在升结肠和乙状结肠黏膜中观察到对5-HT的脱敏现象。使用选择性5-HT拮抗剂，我们已表明5-HT通过5-HT2A受体在乙状结肠中诱导分泌。在升结肠中，5-HT2A和5-HT4受体的组合似乎参与其中。
