冬虫夏草作为一种免疫调节剂。

Cordyceps sinensis as an immunomodulatory agent.

作者信息

Kuo Y C, Tsai W J, Shiao M S, Chen C F, Lin C Y

机构信息

National Research Institute of Chinese Medicine, Veterans General Hospital, Taipei, Taiwan.

出版信息

Am J Chin Med. 1996;24(2):111-25. doi: 10.1142/S0192415X96000165.

DOI:10.1142/S0192415X96000165

PMID:8874668

Abstract

Effects of various fractions of methanol extracts from fruiting bodies of Cordyceps sinensis on the lymphoproliferative response, natural killer (NK) cell activity, and phytohemagglutinin (PHA) stimulated interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha) production on human mononuclear cells (HMNC) were studied. Two of the 15 column fractions (CS-36-39 and CS-48-51) significantly inhibited the blastogenesis response (IC50 = 71.0 +/- 3.0 and 21.7 +/- 2.0 micrograms/ml, respectively), NK cell activity (IC50 = 25.0 +/- 2.5 and 12.9 +/- 5.8 micrograms/ml, respectively) and IL-2 production of HMNC stimulated by PHA (IC50 = 9.6 +/- 2.3 and 5.5 +/- 1.6 micrograms/ml, respectively). TNF-alpha production in HMNC cultures was also blocked by CS-36-39 and CS-48-51 (IC50 = 2.7 +/- 1.0 and 12.5 +/- 3.8 micrograms/ml, respectively). These results indicated that neither CS-36-39 nor CS-48-51 was cytotoxic on HMNC, and that immunosuppressive ingredients are contained in Cordyceps sinensis.

摘要

研究了中华虫草子实体甲醇提取物的不同组分对人单核细胞（HMNC）的淋巴细胞增殖反应、自然杀伤（NK）细胞活性、植物血凝素（PHA）刺激的白细胞介素-2（IL-2）和肿瘤坏死因子-α（TNF-α）产生的影响。15个柱层析组分中的两个（CS-36-39和CS-48-51）显著抑制了母细胞形成反应（IC50分别为71.0±3.0和21.7±2.0微克/毫升）、NK细胞活性（IC50分别为25.0±2.5和12.9±5.8微克/毫升）以及PHA刺激的HMNC的IL-2产生（IC50分别为9.6±2.3和5.5±1.6微克/毫升）。CS-36-39和CS-48-51也阻断了HMNC培养物中TNF-α的产生（IC50分别为2.7±1.0和12.5±3.8微克/毫升）。这些结果表明，CS-36-39和CS-48-51对HMNC均无细胞毒性，且中华虫草中含有免疫抑制成分。