中药蝉花提取物的免疫调节功能

Immunomodulatory functions of extracts from the Chinese medicinal fungus Cordyceps cicadae.

作者信息

Weng Shu-Cheng, Chou Cheng-Jen, Lin Lie-Chwen, Tsai Wei-Jern, Kuo Yuh-Chi

机构信息

Institute of Biology, Fu-Jen University, Taipei, Taiwan, ROC.

出版信息

J Ethnopharmacol. 2002 Nov;83(1-2):79-85. doi: 10.1016/s0378-8741(02)00212-x.

DOI:10.1016/s0378-8741(02)00212-x

PMID:12413710

Abstract

The effects of Cordyceps cicadae extracted fractions on human mononuclear cells (HMNC) proliferation were determined by tritiated thymidine uptake. The results indicated that aqueous methanol (50%) extracts of C. cicadae ascocarps portion (CC-1-2) enhanced HMNC proliferation activated with phytohemagglutinin (PHA) with an EC(50) of 13.8+/-4.6 micro g/ml. By contrast, the methanol (100%) extracts of C. cicadae insect-body portion (CC-2-1) suppressed HMNC proliferation stimulated by PHA with an IC(50) of 32.5+/-5.2 micro g/ml. Cell viability test indicated that inhibitory effects of CC-2-1 on HMNC proliferation were not through direct cytotoxicity. The action mechanisms of CC-1-2 and CC-2-1 may involve the regulation of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) production in HMNC. Since CC-2-1 suppressed IL-2 and IFN-gamma production in HMNC induced with PHA. The CC-1-2 enhanced IL-2 and IFN-gamma production of HMNC stimulated with PHA in a concentration dependent manner. Therefore, the results demonstrated that C. cicadae contained growth modulators for HMNC.

摘要

采用氚标记胸腺嘧啶核苷摄取法测定蝉花提取物对人单核细胞（HMNC）增殖的影响。结果表明，蝉花子座部分的50%甲醇水溶液提取物（CC - 1 - 2）可增强经植物血凝素（PHA）激活的HMNC增殖，其半数有效浓度（EC50）为13.8±4.6μg/ml。相比之下，蝉花虫体部分的100%甲醇提取物（CC - 2 - 1）可抑制PHA刺激的HMNC增殖，其半数抑制浓度（IC50）为32.5±5.2μg/ml。细胞活力测试表明，CC - 2 - 1对HMNC增殖的抑制作用并非通过直接细胞毒性。CC - 1 - 2和CC - 2 - 1的作用机制可能涉及对HMNC中白细胞介素 - 2（IL - 2）和干扰素 - γ（IFN - γ）产生的调节。因为CC - 2 - 1可抑制PHA诱导的HMNC中IL - 2和IFN - γ的产生。而CC - 1 - 2可呈浓度依赖性增强PHA刺激的HMNC中IL - 2和IFN - γ的产生。因此，结果表明蝉花含有HMNC的生长调节剂。