Vagnoni K E, Schram B R, Ginther O J, Lunn D P
Department of Obstetrics and Gynecology, Meriter Hospital, University of Wisconsin, Madison 53715, USA.
Am J Reprod Immunol. 1996 Sep;36(3):184-90. doi: 10.1111/j.1600-0897.1996.tb00160.x.
Equine chorionic girdle cells are a subpopulation of highly invasive trophoblast cells that attach and invade the uterine epithelium on Day 35 (Day 0 = day of ovulation). These invading chorionic girdle cells form endometrial cups that are associated with a marked local maternal leukocytic response that may result in the demise of the cups at Day 120 of pregnancy. Once endometrial cups become established in the uterine wall they do not express MHC antigens, and therefore may only be susceptible to non-MHC restricted cytotoxic cells. The susceptibility of cultured chorionic girdle cells to LAK cell cytotoxicity was tested in order to evaluate the role of this type of cytotoxicity in the life-cycle of endometrial cup tissue.
Chorionic girdle cells from ten Day 34 conceptuses were collected, cultured, and used as a target cell in a lymphokine activated killer assay to determine if these cells were susceptible to lymphokine activated killing.
Cultured chorionic girdle cells demonstrated similar in vitro morphological features and patterns of antigen expression to those seen in vivo in endometrial cups. Cultured chorionic girdle cells were susceptible to lymphokine activated killing.
Lymphokine activated killer cells may play a role in the degeneration of endometrial cup tissue. The potential mechanisms of regulation of uterine LAK cell activity in the horse are discussed.
马绒毛膜带细胞是一类具有高度侵袭性的滋养层细胞亚群，在妊娠第35天（第0天=排卵日）附着并侵入子宫上皮。这些侵入的绒毛膜带细胞形成子宫内膜杯，其与显著的局部母体白细胞反应相关，这可能导致妊娠第120天时子宫内膜杯消失。一旦子宫内膜杯在子宫壁中形成，它们就不表达MHC抗原，因此可能仅对非MHC限制性细胞毒性细胞敏感。为了评估这种细胞毒性类型在子宫内膜杯组织生命周期中的作用，对培养的绒毛膜带细胞对LAK细胞细胞毒性的敏感性进行了测试。
收集来自10个妊娠第34天胚胎的绒毛膜带细胞，进行培养，并在淋巴细胞激活的杀伤试验中用作靶细胞，以确定这些细胞是否易受淋巴细胞激活杀伤的影响。
培养的绒毛膜带细胞在体外表现出与体内子宫内膜杯中所见相似的形态特征和抗原表达模式。培养的绒毛膜带细胞易受淋巴细胞激活杀伤的影响。
淋巴细胞激活的杀伤细胞可能在子宫内膜杯组织的退化中起作用。讨论了马子宫LAK细胞活性调节的潜在机制。
