Mills P C, Roberts C A, Smith N C
Equine Centre, Animal Health Trust, New-Market, Suffolk, England.
Am J Vet Res. 1996 Sep;57(9):1359-63.
To investigate the effects of ozone and airway inflammation on indices of oxidant injury in horses.
5 clinically normal horses and 25 horses referred for poor performance.
Blood, tracheal wash, and bronchoalveolar lavage fluid samples were collected before and after ozone exposure (n = 5) or from clinical cases (n = 25), and were analyzed for reduced glutathione (GSH), glutathione disulfide (GSSG), and free and total iron (Fe) values. A scoring system (0 to 5) was used to assess airway inflammation on the basis of clinical signs and cytologic analysis of the tracheal wash and bronchoalveolar lavage fluid samples.
Ozone induced significant (P < 0.05) increases in GSH (195.4 +/- 68.5 microM), GSSG (19.4 +/- 6.4 microM), and free (25.5 +/- 16.1 microM) and total (93.1 +/- 13.4 microM) Fe values in the pulmonary epithelial lining fluid, compared with preozone samples (49.2 +/- 18.6, 2.4 +/- 1.2, 0.0, and 33.1 +/- 5.9 microM, respectively). The presence of airway inflammation (19/25) was associated with high GSSG and free and total Fe, but not GSH, values in epithelial lining fluid, compared with values for clinically normal horses (6/25). There were no differences in the systemic values of GSH, GSSG, and free and total Fe between any of the groups. A strong correlation (r = 0.84; P < 0.001) existed between inflammation score and the glutathione redox ratio (GSSG/[GSH + GSSG]) in the 25 horses admitted for clinical examination.
Oxidant injury in the lung will induce changes in the glutathione status and Fe homeostasis that could affect pathogenesis of the disease.
Measurement of indices of oxidant injury may be useful in the diagnosis of airway inflammation and the response to inhaled oxidants.
研究臭氧和气道炎症对马氧化损伤指标的影响。
5匹临床正常的马和25匹因表现不佳而转诊的马。
在臭氧暴露前后（n = 5）或从临床病例（n = 25）中采集血液、气管冲洗液和支气管肺泡灌洗液样本，分析其中还原型谷胱甘肽（GSH）、氧化型谷胱甘肽（GSSG）以及游离铁和总铁（Fe）的值。基于临床症状以及气管冲洗液和支气管肺泡灌洗液样本的细胞学分析，使用评分系统（0至5分）评估气道炎症。
与臭氧暴露前的样本（分别为49.2±18.6、2.4±1.2、0.0和33.1±5.9微摩尔）相比，臭氧使肺上皮衬液中的GSH（195.4±68.5微摩尔）、GSSG（19.4±6.4微摩尔）、游离铁（25.5±16.1微摩尔）和总铁（93.1±13.4微摩尔）值显著升高（P < 0.05）。与临床正常马（6/25）相比，存在气道炎症的马（19/25）的上皮衬液中GSSG、游离铁和总铁值较高，但GSH值无差异。各组之间GSH、GSSG以及游离铁和总铁的全身水平无差异。在接受临床检查的25匹马中，炎症评分与谷胱甘肽氧化还原比（GSSG/[GSH + GSSG]）之间存在强相关性（r = 0.84；P < 0.001）。
肺中的氧化损伤会导致谷胱甘肽状态和铁稳态发生变化，这可能影响疾病的发病机制。
氧化损伤指标的测量可能有助于气道炎症的诊断以及对吸入氧化剂的反应评估。
