Concannon P W, Gimpel T, Newton L, Castracane V D
Department of Physiology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Am J Vet Res. 1996 Sep;57(9):1382-5.
To determine time courses of pregnancy-related changes in plasma fibrinogen, serum relaxin, and serum C-reactive protein-like immunoreactivity (CRP-LI) concentrations in pregnant and nonpregnant dogs.
16 pregnant and 13 nonpregnant Beagles, 6 pregnant Beagles, and 3 pregnant and 3 nonpregnant dogs of other breeds.
Fibrinogen was measured by nephelometric centrifugal analysis of light scatter in plasma samples of all dogs, and CRP-LI was measured by fluorescence immunoassay in selected sera from 10 pregnant and 10 nonpregnant dogs. Relaxin was measured in selected sera from 7 pregnant and 7 nonpregnant dogs.
During pregnancy, fibrinogen concentration increased between days 21 and 30 after the luteinizing hormone surge, and was > 280 mg/dl between days 29 and 50 in 84 of 86 samples, with peak values 539 +/- 29 mg/ dl higher (P < 0.01) than those in nonpregnant dogs (188 +/- 8 mg/dl), higher values from days 21 to 50 (P < 0.01) than those in nonpregnant dogs, and values similar to those in nonpregnant dogs after day 50. Relaxin concentration was increased from days 26 to 30 and 0 to 4 days after fibrinogen concentration increased in pregnant dogs, and was not detectable in nonpregnant dogs. The CRP-LI concentration was higher in pregnant dogs at days 26 to 50, but values were inconsistent within and among dogs, and near the limit of sensitivity of the assay.
Concentration of the acute-phase reactant protein fibrinogen increases after day 20 following implantation in dogs, at or just before the pregnancy-specific increase in relaxin concentration from days 26 to 30, and persists until day 50.
Fibrinogen assay may be useful for pregnancy diagnosis in dogs.
确定怀孕和未怀孕犬血浆纤维蛋白原、血清松弛素及血清C反应蛋白样免疫反应性（CRP-LI）浓度与妊娠相关的变化时间进程。
16只怀孕和13只未怀孕的比格犬、6只怀孕的比格犬以及3只怀孕和3只未怀孕的其他品种犬。
对所有犬的血浆样本进行散射比浊离心分析以测量纤维蛋白原，对10只怀孕和10只未怀孕犬的选定血清进行荧光免疫测定以测量CRP-LI。对7只怀孕和7只未怀孕犬的选定血清测量松弛素。
在怀孕期间，黄体生成素激增后第21至30天纤维蛋白原浓度升高，86份样本中的84份在第29至50天>280mg/dl，峰值比未怀孕犬（188±8mg/dl）高539±29mg/dl（P<0.01），第21至50天的值比未怀孕犬高（P<0.01），第50天后的值与未怀孕犬相似。怀孕犬中，松弛素浓度在第26至30天升高，在纤维蛋白原浓度升高后的0至4天升高，未怀孕犬中未检测到。怀孕犬在第26至50天CRP-LI浓度较高，但犬只个体内和个体间的值不一致，且接近检测方法的灵敏度极限。
植入后第20天之后，犬体内急性期反应蛋白纤维蛋白原浓度升高，此时或恰好在第26至30天松弛素浓度出现特定于妊娠的升高之前，并持续至第50天。
纤维蛋白原检测可能有助于犬的妊娠诊断。
