Neuber K, Kröger S, Gruseck E, Abeck D, Ring J
Department of Dermatology and Allergology, University Hospital Hamburg-Eppendort, Germany.
Arch Dermatol Res. 1996 Aug;288(9):532-6.
The aetiology of seborrhoeic dermatitis (SD) is still unknown. An increased number of Pityrosposurm ovale in lesional skin of patients with SD has been suggested to play a crucial role in the pathogenesis of the disease since double-blind trials with antifungal drugs (e.g. ketoconazole) have shown that these agents result in a significantly reduced disease intensity. The frequent association of HIV infection and SD may be due to a suppressed cell-mediated immunity. In order to characterize the role of the humoral and cellular immune response in patients with SD the effects of a P. ovale extract on the proliferation of, and interleukin-2 (IL-2), IL-10 by an interferon-gamma (IFN gamma) production, and immunoglobulin (IgA, IgG, IgM) synthesis by peripheral blood mononuclear cells (PBMC) from patients with SD were studied in vitro. Healthy volunteers served as controls. PBMC from normal donors responded with a significantly increased proliferation to P. ovale antigen, whereas cells from patients with SD did not. Additionally, IL-2 and IFN gamma production by PBMC from patients with SD was markedly depressed compared with normal cells after stimulation with P. ovale. However, stimulation of PBMC from SD patients with P. ovale antigen induced significantly increased IL-10 synthesis. IgA, IgG and IgM synthesis was significantly increased in cultures of PBMC from patients with SD whether the cells were antigen-stimulated or not. Our results support the assumption that strong skin colonization with P. ovale in SD is due to an altered cellular immunity, which may be induced by increased IL-10 secretion.
脂溢性皮炎（SD）的病因仍不清楚。由于抗真菌药物（如酮康唑）的双盲试验表明这些药物可显著降低疾病严重程度，因此有人提出卵圆形糠秕孢子菌在SD患者皮损中的数量增加在该病发病机制中起关键作用。HIV感染与SD的频繁关联可能是由于细胞介导的免疫受到抑制。为了明确体液免疫和细胞免疫反应在SD患者中的作用，我们在体外研究了卵圆形糠秕孢子菌提取物对SD患者外周血单个核细胞（PBMC）增殖、白细胞介素-2（IL-2）和IL-10产生、干扰素-γ（IFNγ）产生以及免疫球蛋白（IgA、IgG、IgM）合成的影响。健康志愿者作为对照。正常供体的PBMC对卵圆形糠秕孢子菌抗原的增殖反应显著增加，而SD患者的细胞则无此反应。此外，与正常细胞相比，卵圆形糠秕孢子菌刺激后，SD患者PBMC产生的IL-2和IFNγ明显降低。然而，用卵圆形糠秕孢子菌抗原刺激SD患者的PBMC可显著增加IL-10的合成。无论是否受到抗原刺激，SD患者PBMC培养物中IgA、IgG和IgM的合成均显著增加。我们的结果支持这样一种假设，即SD中卵圆形糠秕孢子菌在皮肤的大量定植是由于细胞免疫改变所致，这可能是由IL-10分泌增加所诱导的。
