Finn P E, Purnell P, Pilkington G J
Department of Neuropathology, Institute of Psychiatry, London, UK.
Neuropathol Appl Neurobiol. 1996 Aug;22(4):317-24. doi: 10.1111/j.1365-2990.1996.tb01110.x.
Histamine is known to act, at least in part, as a growth factor, as production of this neurotransmitter has been found to accelerate the rate of tissue proliferation in wound repair, embryogenesis and malignant growth. Histamine favours in vivo tumour cell proliferation via H2 receptors. Cimetidine is an H2 blocker and has been shown to inhibit tumour cell growth. In the present study, the growth modulating effects of histamine and cimetidine were assessed on five cell lines derived from human brain tumours of different histological types and grades of malignancy. Each cell line was treated with either cimetidine or histamine for 24 h before kinetic analyses, with PCNA, or motility assays, using Transwell migration chambers incorporating a microporous membrane, were carried out. Cimetidine significantly inhibited cell proliferation in three out of the five cell lines, which may indicate the dependence of proliferation of these cell lines on stimulation of the H2 receptor. With regard to migration, it was observed that in the majority of cell lines, cimetidine induced migration whilst histamine inhibited it. It was concluded that the link between effects of histamine on proliferation and its effects on migration must be clarified using a larger sample of cell lines.
已知组胺至少部分作为一种生长因子发挥作用，因为已发现这种神经递质的产生会加速伤口修复、胚胎发生和恶性生长过程中的组织增殖速率。组胺通过H2受体促进体内肿瘤细胞增殖。西咪替丁是一种H2阻滞剂，已被证明可抑制肿瘤细胞生长。在本研究中，评估了组胺和西咪替丁对源自不同组织学类型和恶性程度等级的人脑肿瘤的五种细胞系的生长调节作用。在进行动力学分析（使用增殖细胞核抗原）或运动性分析（使用装有微孔膜的Transwell迁移小室）之前，每种细胞系用西咪替丁或组胺处理24小时。西咪替丁在五种细胞系中的三种中显著抑制细胞增殖，这可能表明这些细胞系的增殖依赖于H2受体的刺激。关于迁移，观察到在大多数细胞系中，西咪替丁诱导迁移而组胺抑制迁移。得出的结论是，必须使用更大的细胞系样本阐明组胺对增殖的影响与其对迁移的影响之间的联系。
