用编码糖蛋白D或糖蛋白B的DNA疫苗进行免疫，单独或联合使用，可在单纯疱疹病毒2型疾病的动物模型中诱导保护性免疫。
Immunization with DNA vaccines encoding glycoprotein D or glycoprotein B, alone or in combination, induces protective immunity in animal models of herpes simplex virus-2 disease.
作者信息
McClements W L, Armstrong M E, Keys R D, Liu M A
机构信息
Department of Virus and Cell Biology, Merck Research Laboratories, West Point, PA 19486, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11414-20. doi: 10.1073/pnas.93.21.11414.
Abstract
DNA vaccines expressing herpes simplex virus type 2 (HSV-2) full-length glycoprotein D (gD), or a truncated form of HSV-2 glycoprotein B (gB) were evaluated for protective efficacy in two experimental models of HSV-2 infection. Intramuscular (i.m.) injection of mice showed that each construction induced neutralizing serum antibodies and protected the mice from lethal HSV-2 infection. Dose-titration studies showed that low doses (< or = 1 microgram) of either DNA construction induced protective immunity, and that a single immunization with the gD construction was effective. The two DNAs were then tested in a low-dosage combination in guinea pigs. Immune sera from DNA-injected animals had antibodies to both gD and gB, and virus neutralizing activity. When challenged by vaginal infection with HSV-2, the DNA-immunized animals were significantly protected from primary genital disease.
摘要
在两种单纯疱疹病毒2型（HSV-2）感染实验模型中，对表达HSV-2全长糖蛋白D（gD）或HSV-2糖蛋白B（gB）截短形式的DNA疫苗的保护效力进行了评估。对小鼠进行肌肉注射显示，每种构建体均可诱导产生中和血清抗体，并保护小鼠免受致死性HSV-2感染。剂量滴定研究表明，低剂量（≤1微克）的任何一种DNA构建体均可诱导保护性免疫，并且单次接种gD构建体即有效。然后在豚鼠中对这两种DNA进行低剂量联合测试。来自注射DNA动物的免疫血清具有针对gD和gB的抗体以及病毒中和活性。当通过阴道感染HSV-2进行攻击时，经DNA免疫的动物受到显著保护，免受原发性生殖器疾病的侵害。
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本文引用的文献
1
Induction of protective immunity against herpes simplex virus with DNA encoding the immediate early protein ICP 27.
Viral Immunol. 1995;8(2):53-61. doi: 10.1089/vim.1995.8.53.
2
Nucleic acid vaccine encoding gD2 protects mice from herpes simplex virus type 2 disease.编码gD2的核酸疫苗可保护小鼠免受2型单纯疱疹病毒疾病的侵害。
J Infect Dis. 1996 Mar;173(3):536-41. doi: 10.1093/infdis/173.3.536.
3
DNA immunization against experimental genital herpes simplex virus infection.针对实验性生殖器单纯疱疹病毒感染的DNA免疫接种
J Infect Dis. 1996 Apr;173(4):800-7. doi: 10.1093/infdis/173.4.800.
4
Vaccination of mice with herpes simplex virus type 1 glycoprotein D DNA produces low levels of protection against lethal HSV-1 challenge.用1型单纯疱疹病毒糖蛋白D DNA对小鼠进行疫苗接种，对致死性HSV-1攻击产生的保护水平较低。
Antiviral Res. 1995 Oct;28(2):147-57. doi: 10.1016/0166-3542(95)00045-n.
5
Protection against papillomavirus with a polynucleotide vaccine.用多核苷酸疫苗预防乳头瘤病毒。
J Infect Dis. 1996 Feb;173(2):314-20. doi: 10.1093/infdis/173.2.314.
6
Gene inoculation generates immune responses against human immunodeficiency virus type 1.基因接种可引发针对1型人类免疫缺陷病毒的免疫反应。
Proc Natl Acad Sci U S A. 1993 May 1;90(9):4156-60. doi: 10.1073/pnas.90.9.4156.
7
Heterologous protection against influenza by injection of DNA encoding a viral protein.通过注射编码病毒蛋白的DNA实现对流感的异源保护。
Science. 1993 Mar 19;259(5102):1745-9. doi: 10.1126/science.8456302.
8
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J Clin Invest. 1993 Jun;91(6):2837-43. doi: 10.1172/JCI116527.
10
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J Infect Dis. 1993 Sep;168(3):622-9. doi: 10.1093/infdis/168.3.622.
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