Division of Infectious Diseases, School of Medicine, University of California, Irvine, Irvine, California, USA.
J Virol. 2012 Apr;86(8):4328-39. doi: 10.1128/JVI.05194-11. Epub 2012 Feb 8.
Routine serodiagnosis of herpes simplex virus (HSV) infections is currently performed using recombinant glycoprotein G (gG) antigens from herpes simplex virus 1 (HSV-1) and HSV-2. This is a single-antigen test and has only one diagnostic application. Relatively little is known about HSV antigenicity at the proteome-wide level, and the full potential of mining the antibody repertoire to identify antigens with other useful diagnostic properties and candidate vaccine antigens is yet to be realized. To this end we produced HSV-1 and -2 proteome microarrays in Escherichia coli and probed them against a panel of sera from patients serotyped using commercial gG-1 and gG-2 (gGs for HSV-1 and -2, respectively) enzyme-linked immunosorbent assays. We identified many reactive antigens in both HSV-1 and -2, some of which were type specific (i.e., recognized by HSV-1- or HSV-2-positive donors only) and others of which were nonspecific or cross-reactive (i.e., recognized by both HSV-1- and HSV-2-positive donors). Both membrane and nonmembrane virion proteins were antigenic, although type-specific antigens were enriched for membrane proteins, despite being expressed in E. coli.
目前，单纯疱疹病毒（HSV）感染的常规血清学诊断是使用来自单纯疱疹病毒 1（HSV-1）和 HSV-2 的重组糖蛋白 G（gG）抗原进行的。这是一种单抗原检测方法，只有一种诊断应用。关于疱疹病毒在蛋白质组水平上的抗原性，人们相对知之甚少，而充分利用抗体库来识别具有其他有用诊断特性和候选疫苗抗原的抗原，还有待实现。为此，我们在大肠杆菌中制备了 HSV-1 和 -2 蛋白质组微阵列，并使用商业 gG-1 和 gG-2（分别用于 HSV-1 和 -2 的 gGs）酶联免疫吸附试验对来自患者的血清进行了探测。我们在 HSV-1 和 -2 中都鉴定出了许多反应性抗原，其中一些是特定类型的（即仅被 HSV-1 或 HSV-2 阳性供体识别），而另一些是非特异性或交叉反应的（即被 HSV-1 和 HSV-2 阳性供体识别）。包膜和非包膜病毒粒子蛋白都是抗原性的，尽管膜蛋白富含特定类型的抗原，但它们在大肠杆菌中表达。
