Kriesel J D, Spruance S L, Daynes R A, Araneo B A
Department of Medicine, University of Utah School of Medicine; Salt Lake City, 84132, USA.
J Infect Dis. 1996 Mar;173(3):536-41. doi: 10.1093/infdis/173.3.536.
Nucleic acid vaccinations with plasmids pWW65, containing the sequence for herpes simplex type 2 (HSV-2) gD2, and pRSVnt, lacking the gD sequence, were studied. Groups of mice were immunized with pWW65 alone, pWW65 plus 1,25-dihydroxyvitamin-D3 (D3), or pRSVnt. Clinical disease (vaginitis), serum and vaginal washing antibody levels, and vaginal washing virus titers were measured intravaginal HSV-2 challenge. No animals (0/10) in the pWW65 + D3 group, 6/10 animals in the pWW65 group, and 10/10 animals in the pRSVnt group developed severe disease by postchallenge day 13 (P<.001, P=.04 vs. pRSVnt). Virus titers in vaginal washings were significantly reduced in the pWW65 and pWW65+D3 groups versus the pRSVnt group (P<.001). Increasing levels of serum anti-gD2 antibodies were measured 2 and 6 days after challenge among animals in the pWW65 and pWW65+D3 groups but not among animals in the pRSVnt group. Vaccinations with a plasmid containing the gD2 gene are immunogenic and provide some protection from HSV-2-induced disease.
对含有2型单纯疱疹病毒（HSV-2）gD2序列的质粒pWW65和缺乏gD序列的质粒pRSVnt进行了核酸疫苗研究。将小鼠分组，分别用单独的pWW65、pWW65加1,25-二羟基维生素D3（D3）或pRSVnt进行免疫。在阴道内进行HSV-2攻击后，测量临床疾病（阴道炎）、血清和阴道冲洗液抗体水平以及阴道冲洗液病毒滴度。在攻击后第13天，pWW65 + D3组中无动物（0/10）、pWW65组中有6/10动物、pRSVnt组中有10/10动物出现严重疾病（P<.001，pWW65组与pRSVnt组相比P=.04）。与pRSVnt组相比，pWW65组和pWW65 + D3组阴道冲洗液中的病毒滴度显著降低（P<.001）。在攻击后2天和6天，pWW65组和pWW65 + D3组动物血清中抗gD2抗体水平升高，而pRSVnt组动物血清中抗gD2抗体水平未升高。用含有gD2基因的质粒进行疫苗接种具有免疫原性，并能为HSV-2诱导的疾病提供一定保护。
