Vacca-Smith A M, Venkitaraman A R, Schilling K M, Bowen W H
Department of Dental Research, Rochester Caries Research Center, University of Rochester, NY 14642, USA.
Caries Res. 1996;30(5):354-60. doi: 10.1159/000262342.
Activities of glucosyltransferases (Gtf)-B, -C and -D adsorbed on the surface of saliva-coated hydroxyapatite were compared with those of Gtfs of donor whole saliva. Antiserum raised against a mixture of the three Gtfs reduced the activity of GtfB only, and had no effect on donor Gtf activities. GtfB, and not the Gtfs of the donors, was stimulated in the presence of starch hydrosylates. GtfD and GtfC activities were enhanced on the surfaces, as were the Gtf activities of donor salivas. The activities of GtfD and GtfB, but not GtfC, were stimulated by dextran. The donor Gtf activities were unaffected by dextran. Therefore, Gtf activity in pellicles has properties similar to those of GtfC.
将吸附在唾液包被的羟基磷灰石表面的葡糖基转移酶（Gtf）-B、-C和-D的活性与供体全唾液中Gtf的活性进行了比较。针对三种Gtf混合物产生的抗血清仅降低了GtfB的活性，对供体Gtf活性没有影响。在存在淀粉水解产物的情况下，GtfB（而非供体的Gtf）受到刺激。GtfD和GtfC的活性在表面增强，供体唾液的Gtf活性也是如此。葡聚糖刺激了GtfD和GtfB的活性，但未刺激GtfC的活性。供体Gtf活性不受葡聚糖影响。因此，菌斑中的Gtf活性具有与GtfC相似的特性。
