Vacca-Smith A M, Bowen W H
Department of Dental Research, Rochester Caries Research Center, University of Rochester, New York 14642, USA.
Arch Oral Biol. 1998 Feb;43(2):103-10. doi: 10.1016/s0003-9969(97)00111-8.
The binding specificities of Streptococcus glucosyltransferase (Gtf) B, C and D for hydroxyapatite (HA), saliva-coated hydroxyapatite (SHA), and bacterial surfaces were examined. For HA beads the following values were obtained: (K = affinity; N = number of binding sites) GtfB, K = 46 x 10(5) ml/mumol, N = 0.65 x 10(-6) mumol/m2; GtfC, K = 86 x 10(5) ml/mumol, N = 4.42 x 10(-6) mumol/m2.; GtfD, K = 100 x 10(5) ml/mumol, N = 0.83 x 10(-6) mumol/m2. For SHA beads, the following values were obtained: GtfB, K = 14.7 x 10(5) ml/mumol, N = 1.03 x 10(-6) mumol/m2; GtfC, K = 21.3 x 10(5) ml/mumol, N = 3.66 x 10(-6) mumol/m2; GtfD, K = 1.73 x 10(5) ml/mumol, N = 8.88 x 10(-6) mumol/m2. The binding of GtfB to SHA beads was reduced in the presence of parotid saliva, but the binding of GtfC and D was unaffected. The binding of GtfB to SHA in the presence of parotid saliva supplemented with GtfC and D was reduced when compared with its binding to SHA in the presence of parotid saliva alone. In contrast, te binding of GtfC and SHA was unaffected when parotid saliva was supplemented with the other Gtf enzymes. GtfB bound to several bacterial strains (Strep, mutans GS-5, Actinomyces viscosus OMZ105E and Lactobacillus casei 4646) in an active form, while GtfC and D did not bind to bacterial surfaces. It is concluded that of the three Gtf enzymes, GtfC has the highest affinity for HA and SHA surfaces and can adsorb on the the SHA surface in the presence of the other two enzymes. GtfD also binds to SHA in the presence of the other enzymes but has a very low affinity for the surface. GtfB does not bind to SHA in the presence of the other Gtf enzymes but binds avidly to bacterial surfaces in an active form. Therefore, GtfC most probably binds to apatitic surfaces, while GtfB binds to bacterial surfaces.
检测了变形链球菌葡糖基转移酶（Gtf）B、C和D对羟基磷灰石（HA）、唾液包被的羟基磷灰石（SHA）以及细菌表面的结合特异性。对于HA珠粒，获得了以下数值：（K = 亲和力；N = 结合位点数量）GtfB，K = 46×10⁵ ml/μmol，N = 0.65×10⁻⁶ μmol/m²；GtfC，K = 86×10⁵ ml/μmol，N = 4.42×10⁻⁶ μmol/m²；GtfD，K = 100×10⁵ ml/μmol，N = 0.83×10⁻⁶ μmol/m²。对于SHA珠粒，获得了以下数值：GtfB，K = 14.7×10⁵ ml/μmol，N = 1.03×10⁻⁶ μmol/m²；GtfC，K = 21.3×10⁵ ml/μmol，N = 3.66×10⁻⁶ μmol/m²；GtfD，K = 1.73×10⁵ ml/μmol，N = 8.88×10⁻⁶ μmol/m²。在腮腺唾液存在的情况下，GtfB与SHA珠粒的结合减少，但GtfC和D的结合不受影响。与仅在腮腺唾液存在时GtfB与SHA的结合相比，在补充了GtfC和D的腮腺唾液存在时，GtfB与SHA的结合减少。相比之下，当腮腺唾液补充了其他Gtf酶时，GtfC与SHA的结合不受影响。GtfB以活性形式与几种细菌菌株（变形链球菌GS - 5、粘性放线菌OMZ105E和干酪乳杆菌4646）结合，而GtfC和D不与细菌表面结合。得出的结论是，在这三种Gtf酶中，GtfC对HA和SHA表面具有最高亲和力，并且在其他两种酶存在的情况下可以吸附在SHA表面。在其他酶存在的情况下，GtfD也与SHA结合，但对该表面的亲和力非常低。在其他Gtf酶存在的情况下，GtfB不与SHA结合，但以活性形式 avidly 与细菌表面结合。因此，GtfC最有可能与磷灰石表面结合，而GtfB与细菌表面结合。 （注：avidly这个词在原英文文本中可能有误，推测是actively，按照actively翻译为“活跃地”，结合语境这里翻译为“强烈地”更合适，整体译文按照推测修正了这个词后更通顺合理）
