Asakura E, Hanamura T, Umemura A, Yada K, Yamauchi T, Tanabe T
Central Research Laboratory, Green Cross Corporation, Osaka, Japan.
Immunobiology. 1996 Aug;195(3):300-13. doi: 10.1016/S0171-2985(96)80047-7.
M-CSF is a macrophage-lineage-specific growth factor that causes proliferation and differentiation of progenitor cells in the bone marrow. To investigate the effects of M-CSF on more matured cells, human monocytes were cultured in the presence or absence of M-CSF for 6 days. Addition of M-CSF at more than 10(2) U/ml resulted in higher viability and caused morphological differentiation to large macrophage-like cells. LPS-induced mediator production was also compared between M-CSF-treated and control cell. Monocytes were incubated with or without M-CSF for 3 days, and were stimulated with 1 microgram/ml of LPS for 2 days. IL-1 beta was not detected in the both culture supernatants, and PGE2 production was not influenced by M-CSF. However, amounts of G-CSF, GM-CSF, IL-6, and TNF-alpha produced in response to 1 microgram/ml of LPS were 1.5 to 2 times greater from monocytes treated with 10(4) U/ml of M-CSF than from control cells. The priming effect of M-CSF on LPS-induced cytokine production was found to require 3-day preincubation, and reached a maximum at the concentration of 10(4) U/ml. M-CSF-treated cells responded to a 10 times lower concentration of LPS than control cells in terms of cytokine production. M-CSF was also shown by flowcytometric analysis to influence the expression of CD14, a receptor for LPS, which might render monocytes more sensitive to LPS.
巨噬细胞集落刺激因子（M-CSF）是一种巨噬细胞谱系特异性生长因子，可促使骨髓中的祖细胞增殖和分化。为了研究M-CSF对更成熟细胞的影响，将人单核细胞在有或无M-CSF的情况下培养6天。添加浓度超过10² U/ml的M-CSF可提高细胞活力，并导致其形态分化为大型巨噬细胞样细胞。同时还比较了M-CSF处理组和对照组细胞中脂多糖（LPS）诱导的介质产生情况。将单核细胞在有或无M-CSF的情况下孵育3天，然后用1微克/毫升的LPS刺激2天。在两种培养上清液中均未检测到白细胞介素-1β（IL-1β），且前列腺素E2（PGE2）的产生不受M-CSF影响。然而，对于1微克/毫升的LPS刺激，用10⁴ U/ml的M-CSF处理的单核细胞产生的粒细胞集落刺激因子（G-CSF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、IL-6和肿瘤坏死因子-α（TNF-α）的量比对照细胞多1.5至2倍。发现M-CSF对LPS诱导的细胞因子产生的启动作用需要预孵育3天，且在浓度为10⁴ U/ml时达到最大值。就细胞因子产生而言，M-CSF处理的细胞对LPS浓度的反应比对照细胞低10倍。流式细胞术分析还显示，M-CSF会影响LPS受体CD14的表达，这可能使单核细胞对LPS更敏感。
