Wieczorowska-Tobis K, Breborowicz A, Witowski J, Martis L, Oreopoulos D G
Pathophysiology Department, Medical School Poznań, Poland.
J Physiol Pharmacol. 1996 Sep;47(3):535-43.
Because of the evidence that peritoneal macrophages are activated during peritoneal dialysis, we hypothesised that the injury of the peritoneum is, at least in part, dependent on the intraperitoneal generation of free radicals. The aim of the study was to evaluate the effect of vitamin E on the peroxidation and permeability of the peritoneum during chronic peritoneal dialysis in rats. Supplementation of the intraperitoneally infused saline with vitamin E decreased the peroxidation of peritoneum estimated as the malondialdehyde (MDA) level in rats' omentum. However the permeability of the peritoneum to glucose and protein in vitamin E treated rats was increased. In in vitro study we have found that vitamin E is cytotoxic to human mesothelial cells (HMC) as measured by inhibition of their proliferation and this effect was irreversible. We conclude that vitamin E, despite its antioxidant effect, causes the changes of the peritoneum permeability which could decrease the effectiveness of peritoneal dialysis.
由于有证据表明腹膜巨噬细胞在腹膜透析过程中被激活，我们推测腹膜损伤至少部分取决于腹膜内自由基的产生。本研究的目的是评估维生素E对大鼠慢性腹膜透析期间腹膜过氧化和通透性的影响。在腹腔内注入的盐水中补充维生素E可降低以大鼠大网膜中丙二醛（MDA）水平估算的腹膜过氧化。然而，维生素E处理的大鼠腹膜对葡萄糖和蛋白质的通透性增加。在体外研究中，我们发现通过抑制人腹膜间皮细胞（HMC）增殖来衡量，维生素E对其具有细胞毒性，且这种作用是不可逆的。我们得出结论，维生素E尽管具有抗氧化作用，但会导致腹膜通透性改变，这可能会降低腹膜透析的有效性。
