Haapala J, Lammi M J, Inkinen R, Parkkinen J J, Agren U M, Arokoski J, Kiviranta I, Helminen H J, Tammi M I
Department of Anatomy, University of Kuopio, Finland.
J Rheumatol. 1996 Sep;23(9):1586-93.
To study the influence of joint loading and immobilization on articular cartilage hyaluronan concentration and histological distribution in the knee joints of young dogs subjected to 11 weeks' immobilization by splinting, and 15 weeks' running exercise at a rate of 40 km/day.
The amount of hyaluronan in articular cartilage was determined by a competitive binding assay using a biotinylated hyaluronan binding complex (HABC) of aggrecan and link protein. Histologic sections were stained for the localization of hyaluronan with the HABC probe. Extracted proteoglycans were characterized by sodium dodecyl sulfate agarose gel electrophoresis.
Immobilization significantly reduced the concentration of hyaluronan in all sites studied (tibial and femoral condyles, patellar surface of femur). The proportion of hyaluronan to total uronic acid (mainly from aggrecan) remained unchanged because of a concurrent decrease in aggrecan. The ratio of hyaluronan and aggrecan remained constant also in runners. The staining pattern of free hyaluronan in the tissue sections and the electrophoretic mobility of the extracted proteoglycans were not affected by the different loading regimes.
Reduced joint loading due to splint immobilization significantly decreases both hyaluronan and aggrecan in the articular cartilage. The remarkably parallel changes in aggrecan and hyaluronan content suggest that joint loading exerts a coordinated influence on their metabolism.
研究关节负荷与固定对幼犬膝关节透明质酸浓度及组织学分布的影响。幼犬分别经历11周夹板固定制动及以每天40公里的速度进行15周跑步运动。
采用生物素化的聚集蛋白聚糖与连接蛋白的透明质酸结合复合物（HABC）通过竞争结合试验测定关节软骨中透明质酸的含量。用HABC探针进行组织学切片染色以定位透明质酸。通过十二烷基硫酸钠琼脂糖凝胶电泳对提取的蛋白聚糖进行表征。
固定显著降低了所有研究部位（胫骨和股骨髁、股骨髌面）的透明质酸浓度。由于聚集蛋白聚糖同时减少，透明质酸占总糖醛酸（主要来自聚集蛋白聚糖）的比例保持不变。在跑步的幼犬中，透明质酸与聚集蛋白聚糖的比例也保持恒定。组织切片中游离透明质酸的染色模式和提取的蛋白聚糖的电泳迁移率不受不同负荷方式的影响。
夹板固定导致的关节负荷减少显著降低了关节软骨中的透明质酸和聚集蛋白聚糖。聚集蛋白聚糖和透明质酸含量的显著平行变化表明关节负荷对它们的代谢产生协同影响。
