猫疱疹病毒1型立即早期基因表达的特征分析

Characterization of the feline herpesvirus type 1 immediate early gene expression.

作者信息

Kawaguchi Y, Maeda K, Ono M, Yokoyama N, Tsubota K, Fujita K, Tohya Y, Kai C, Mikami T

机构信息

Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.

出版信息

J Vet Med Sci. 1996 Aug;58(8):715-21. doi: 10.1292/jvms.58.715.

DOI:10.1292/jvms.58.715

PMID:8877967

Abstract

We analyzed the mechanism for feline herpesvirus type 1 (FHV-1) immediate early (IE) gene expression. We demonstrated that (i) the transcription initiation site of the FHV-1 IE transcript lies in the putative FHV-1 ICP4 binding site, (ii) the FHV-1 IE transcript is spliced to remove 906 bp intron from the leader region, (iii) cis-acting elements of the FHV-1 IE promoter map both down- and upstream of the transcription initiation site, and (iv) a deletion of a 58 bp sequence which includes the putative FHV-1 ICP4 binding site in the IE promoter resulted in significant induction of promoter activity by a FHV-1 IE gene product although the FHV-1 IE gene product slightly stimulates the FHV-1 IE promoter, indicating that the FHV-1 IE gene product down-regulates its own promoter via the region.

摘要

我们分析了猫疱疹病毒1型（FHV-1）立即早期（IE）基因表达的机制。我们证明：（i）FHV-1 IE转录本的转录起始位点位于假定的FHV-1 ICP4结合位点；（ii）FHV-1 IE转录本经过剪接，从先导区去除了906 bp的内含子；（iii）FHV-1 IE启动子的顺式作用元件定位于转录起始位点的下游和上游；（iv）IE启动子中一个包含假定的FHV-1 ICP4结合位点的58 bp序列的缺失，导致FHV-1 IE基因产物对启动子活性有显著诱导作用，尽管FHV-1 IE基因产物对FHV-1 IE启动子有轻微刺激，这表明FHV-1 IE基因产物通过该区域下调其自身启动子。