Kumar S, Green G A, Teruya-Feldstein J, Raffeld M, Jaffe E S
Hematopathology Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1500, USA.
Mod Pathol. 1996 Sep;9(9):925-9.
The CD23 antigen is a low-affinity immunoglubulin E receptor that is expressed during B-cell activation. Recently, it has been shown to be of diagnostic utility in distinguishing between small lymphocytic lymphoma (SLL) and mantle cell lymphoma (MCL), two entities that can have similar morphologic and immunophenotypic features. Such studies, however, generally required viable cells in cell suspension or cryostat sections for detection of CD23. We evaluated staining for the CD23 antigen in paraffin sections, using BU38, an antibody that detects a fixation-resistant epitope of the antigen. We analyzed 44 SLLs, 3 lymphoplasmacytoid lymphomas, and 39 MCLs. Staining was performed on formalin- or B5-fixed paraffin-embedded tissue sections using L26 (CD20), CD3, Leu22 (CD43), and BU38 (CD23) antibodies. All of the cases were of B-cell phenotype (CD20+), and 42/44 SLLs, 3/3 lymphoplasmacytoid lymphomas, and 33/39 MCLs coexpressed the CD43 antigen. CD23 was positive in 41 (93%) of 44 SLLs. The majority of neoplastic cells (75% or more) stained positively, with a membranous pattern of staining. The staining was moderate in intensity and easily interpreted. Only 1/39 MCLs and 1/3 lymphoplasmacytoid lymphomas were CD23 positive. CD23-positive follicular dendritic cells were, however, present in all of the MCLs, either in residual follicles or in large, disordered meshworks. These results demonstrate that the BU38 antibody can detect CD23 on the cells of SLLs in paraffin sections and that this antibody can have diagnostic utility in routine diagnosis.
CD23抗原是一种低亲和力免疫球蛋白E受体，在B细胞活化过程中表达。最近研究表明，它在鉴别小淋巴细胞淋巴瘤（SLL）和套细胞淋巴瘤（MCL）方面具有诊断价值，这两种实体瘤在形态学和免疫表型特征上可能相似。然而，此类研究通常需要细胞悬液或冷冻切片中的活细胞来检测CD23。我们使用BU38（一种能检测该抗原抗固定表位的抗体）评估石蜡切片中CD23抗原的染色情况。我们分析了44例SLL、3例淋巴浆细胞样淋巴瘤和39例MCL。使用L26（CD20）、CD3、Leu22（CD43）和BU38（CD23）抗体对福尔马林或B5固定的石蜡包埋组织切片进行染色。所有病例均为B细胞表型（CD20+），44例SLL中有42例、3例淋巴浆细胞样淋巴瘤中有3例、39例MCL中有33例共表达CD43抗原。44例SLL中有41例（93%）CD23呈阳性。大多数肿瘤细胞（75%或更多）染色呈阳性，呈膜性染色模式。染色强度适中，易于解读。39例MCL中只有1例、3例淋巴浆细胞样淋巴瘤中只有1例CD23呈阳性。然而，所有MCL中均存在CD23阳性的滤泡树突状细胞，要么存在于残留滤泡中，要么存在于大的、无序的网络中。这些结果表明，BU38抗体可在石蜡切片中检测SLL细胞上的CD23，且该抗体在常规诊断中具有诊断价值。
