Hurley M J, Stubbs C M, Jenner P, Marsden C D
Neurodegenerative Disease Research Centre, King's College London, UK.
Neurosci Lett. 1996 Aug 23;214(2-3):75-8. doi: 10.1016/0304-3940(96)12884-6.
Quantitative receptor autoradiography and in situ hybridization were used to investigate the expression of dopamine D3 receptors in sections of human brain containing limbic (nucleus accumbens) and striatal (caudate nucleus, putamen) regions. High levels of dopamine D3 receptor mRNA and specific 3H7-hydroxy-N,N-di-N-propyl-2-aminotetralin ([3H]7-OH-DPAT) binding sites were detected in the nucleus accumbens with lower levels in the caudate nucleus and putamen. No difference in D3 receptor expression was observed between normal and parkinsonian brain. These results indicate that D3 receptor expression is not altered in Parkinson's disease. In addition, they suggest that dopamine release in striatal and limbic areas is not necessary for maintenance of D3 receptor expression.
采用定量受体放射自显影术和原位杂交技术，研究多巴胺D3受体在包含边缘系统（伏隔核）和纹状体（尾状核、壳核）区域的人脑切片中的表达情况。在伏隔核中检测到高水平的多巴胺D3受体mRNA和特异性3H7-羟基-N,N-二-N-丙基-2-氨基四氢萘（[3H]7-OH-DPAT）结合位点，在尾状核和壳核中的水平较低。在正常脑和帕金森病脑之间未观察到D3受体表达的差异。这些结果表明，帕金森病中D3受体表达未发生改变。此外，它们提示，纹状体和边缘系统区域的多巴胺释放对于维持D3受体表达并非必需。
