Nave R, Herer P, Haimov I, Shlitner A, Lavie P
Sleep Laboratory, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Neurosci Lett. 1996 Aug 23;214(2-3):123-6. doi: 10.1016/0304-3940(96)12899-8.
In this double-blind, placebo-controlled study we investigated whether 10 mg flumazenil, a pure benzodiazepine antagonist, can block the hypnotic and hypothermic effects of 3 mg melatonin. The design comprised four 7-h (1200-1900 h) testing periods, preceded by a 'no-treatment' adaptation period of the "7/13' sleep-wake paradigm. Six young healthy adult males were paid to participate. During each experimental period, tablets were administered at 1145 h (flumazenil or placebo) and at 1200 h (melatonin or placebo) in a randomized, double-blind, partially repeated Latin square design. Polysomnographic recordings and core body temperature recordings revealed that melatonin, either in combination with placebo or with flumazenil, significantly increased the amounts of sleep, and decreased core body temperature in comparison with placebo alone or the combination of flumazenil plus placebo. These results do not support the hypothesis that melatonin exerts its hypothermic and hypnotic effects via the central benzodiazepine receptors.
在这项双盲、安慰剂对照研究中，我们调查了10毫克氟马西尼（一种纯苯二氮䓬拮抗剂）是否能够阻断3毫克褪黑素的催眠和降温作用。研究设计包括四个7小时（12:00 - 19:00）的测试期，之前有一个“7/13”睡眠 - 觉醒模式的“无治疗”适应期。六名年轻健康成年男性被招募参与研究并获得报酬。在每个实验期，按照随机、双盲、部分重复拉丁方设计，于11:45服用片剂（氟马西尼或安慰剂），并于12:00服用片剂（褪黑素或安慰剂）。多导睡眠图记录和核心体温记录显示，与单独使用安慰剂或氟马西尼加安慰剂的组合相比，褪黑素无论是与安慰剂联合还是与氟马西尼联合，均显著增加了睡眠时间，并降低了核心体温。这些结果不支持褪黑素通过中枢苯二氮䓬受体发挥其降温及催眠作用的假说。
