定位在9号染色体上的新型人类G蛋白偶联受体（GPCR）基因的分子克隆。

Molecular cloning of the novel human G protein-coupled receptor (GPCR) gene mapped on chromosome 9.

作者信息

Yamaguchi F, Tokuda M, Hatase O, Brenner S

机构信息

Department of Physiology, Faculty of Medicine, Kagawa Medical University, Japan.

出版信息

Biochem Biophys Res Commun. 1996 Oct 14;227(2):608-14. doi: 10.1006/bbrc.1996.1553.

DOI:10.1006/bbrc.1996.1553

PMID:8878560

Abstract

A novel human GPCR gene was cloned by PCR with degenerate primers designed from cannabinoid type 1 receptor (CB1) sequences and a full-length clone was isolated by screening of a human genomic DNA library. This gene, termed EDG-3, is highly homologous (51.9 % overall and 69.2 % in seven transmembrane regions) to human EDG-1, 47.9 % to rat AGR16 but only 28.0 % to human CB1 receptor. The Northern hybridization analysis showed that a 2.8 kb transcript of EDG-3 is abundantly expressed in the heart followed by placenta, kidney and liver. The EDG-3 gene was mapped on the human chromosome 9q22.1-q22.2 by fluorescence in situ hybridization analysis. Although the ligand and physiological role of this receptor is unknown, this gene may be a new member of the EDG family.

摘要

利用从1型大麻素受体（CB1）序列设计的简并引物，通过聚合酶链反应（PCR）克隆了一个新的人类G蛋白偶联受体（GPCR）基因，并通过筛选人类基因组DNA文库分离出一个全长克隆。这个基因被命名为EDG-3，与人类EDG-1高度同源（总体同源性为51.9%，在七个跨膜区域为69.2%），与大鼠AGR16同源性为47.9%，但与人类CB1受体仅为28.0%。Northern杂交分析表明，EDG-3的一个2.8kb转录本在心脏中大量表达，其次是胎盘、肾脏和肝脏。通过荧光原位杂交分析，将EDG-3基因定位在人类染色体9q22.1-q22.2上。尽管该受体的配体和生理作用尚不清楚，但该基因可能是EDG家族的一个新成员。