Bratanov V, Lilkova N, Stoianova-Zaĭkova L
Vet Med Nauki. 1978;15(9):28-33.
Experiments were carried out to fractionate an antigen of Trichinella spiralis with Sephadex G-50 and G-100. The specificty of the fractions has been followed up by means of the intradermal allergic reaction in six pigs, experimentally infected with Trichenalla spiralis, one with Trichocephalus suis and three, born by trichinellosis-affected sows. The second fraction has been tested also on four pigs, experimentally inoculated with Ascaris suum, nine--with Ascarsis suum and Oesophagostomum and 19 healthy pigs. The intradermal allergic tests were effected with thrichinellous fractions, diluted 1:1000. The results obtained from the investigations indicate that the best separation of the complete trichinellous antigen, obtained from lyophilized trichinellae, is achieved with Sephadex G-100. In the case of an intradermal allergic test, a well-expressed reaction is attained with the 2nd fraction and the 1st fraction, the reaction being maintained for a longer times with the 2nd fraction.
开展了用葡聚糖凝胶G - 50和G - 100对旋毛虫抗原进行分级分离的实验。通过对6头经实验感染旋毛虫、1头感染猪鞭虫以及3头由患旋毛虫病母猪所生仔猪进行皮内过敏反应，追踪各分级部分的特异性。还对4头经实验接种猪蛔虫、9头接种猪蛔虫和食道口线虫的猪以及19头健康猪进行了第二分级部分的检测。皮内过敏试验采用稀释1:1000的旋毛虫分级部分进行。研究所得结果表明，用葡聚糖凝胶G - 100能最好地分离从冻干旋毛虫获得的完整旋毛虫抗原。在皮内过敏试验中，第二分级部分和第一分级部分出现明显反应，第二分级部分的反应持续时间更长。
